PAFAH2 Antibody (PACO62803)
- SKU:
- PACO62803
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PAFAH2 Antibody (PACO62803) |
抗体コード: | PACO62803 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Platelet-activating factor acetylhydrolase 2, cytoplasmic protein (1-206AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO62803 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO62803 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme. |
シノニム: | Platelet-activating factor acetylhydrolase 2, cytoplasmic (EC 3.1.1.47) (Serine-dependent phospholipase A2) (SD-PLA2) (hSD-PLA2), PAFAH2 |
UniProt Protein Function: | PAFAH2: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme. Belongs to the serine esterase family. |
UniProt Protein Details: | Protein type:Hydrolase; Lipid-binding; EC 3.1.1.47; Lipid Metabolism - ether lipid Chromosomal Location of Human Ortholog: 1p36 Molecular Function:phospholipid binding Biological Process: lipid metabolic process |
NCBI Summary: | This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q99487 |
NCBI GenInfo Identifier: | 6647691 |
NCBI Gene ID: | 5051 |
NCBI Accession: | Q99487.1 |
UniProt Secondary Accession: | Q99487,O15458, Q5SY02, D3DPK1, |
UniProt Related Accession: | Q99487 |
Molecular Weight: | 44,036 Da |
NCBI Full Name: | Platelet-activating factor acetylhydrolase 2, cytoplasmic |
NCBI Synonym Full Names: | platelet activating factor acetylhydrolase 2 |
NCBI Official Symbol: | PAFAH2 |
NCBI Official Synonym Symbols: | HSD-PLA2 |
NCBI Protein Information: | platelet-activating factor acetylhydrolase 2, cytoplasmic |
UniProt Protein Name: | Platelet-activating factor acetylhydrolase 2, cytoplasmic |
UniProt Synonym Protein Names: | Serine-dependent phospholipase A2; SD-PLA2; hSD-PLA2 |
Protein Family: | Platelet-activating factor acetylhydrolase |
UniProt Gene Name: | PAFAH2 |
UniProt Entry Name: | PAFA2_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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