NUDT15 Antibody (PACO54586)
- SKU:
- PACO54586
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NUDT15 Antibody (PACO54586) |
抗体コード: | PACO54586 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Nucleotide triphosphate diphosphatase NUDT15 protein (1-164AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescent analysis of HepG2 cells using PACO54586 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO54586 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP. Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives. Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear. Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs. May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA. |
シノニム: | Nucleotide triphosphate diphosphatase NUDT15 (EC 3.6.1.9) (MutT homolog 2) (MTH2) (Nucleoside diphosphate-linked moiety X motif 15) (Nudix motif 15) (Nucleoside diphosphate-linked to another moiety X hydrolase 15) (Nudix hydrolase 15), NUDT15, MTH2 |
UniProt Protein Function: | NUDT15: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear. May have a role in DNA synthesis and cell cycle progression through the interaction with PCNA. Belongs to the Nudix hydrolase family.Protein type: Hydrolase; EC 3.6.1.55Chromosomal Location of Human Ortholog: 13q14.2Cellular Component: cytosolMolecular Function: 8-oxo-7,8-dihydroguanine triphosphatase activity; nucleoside-diphosphatase activityBiological Process: dGTP catabolic process |
UniProt Protein Details: | |
NCBI Summary: | This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016] |
UniProt Code: | Q9NV35 |
NCBI GenInfo Identifier: | 68565944 |
NCBI Gene ID: | 55270 |
NCBI Accession: | Q9NV35.1 |
UniProt Secondary Accession: | Q9NV35,Q32Q27, Q6P2C9, A2RUR6 |
UniProt Related Accession: | Q9NV35 |
Molecular Weight: | 18,609 Da |
NCBI Full Name: | Probable 8-oxo-dGTP diphosphatase NUDT15 |
NCBI Synonym Full Names: | nudix hydrolase 15 |
NCBI Official Symbol: | NUDT15 |
NCBI Official Synonym Symbols: | MTH2; NUDT15D |
NCBI Protein Information: | probable 8-oxo-dGTP diphosphatase NUDT15 |
UniProt Protein Name: | Probable 8-oxo-dGTP diphosphatase NUDT15 |
UniProt Synonym Protein Names: | 7,8-dihydro-8-oxoguanine-triphosphatase NUDT15; MutT homolog 2; MTH2; Nucleoside diphosphate-linked moiety X motif 15; Nudix motif 15 |
Protein Family: | Nudix hydrolase |
UniProt Gene Name: | NUDT15 |
UniProt Entry Name: | NUD15_HUMAN |