NPHS1 Antibody (PACO28618)
- SKU:
- PACO28618
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NPHS1 Antibody (PACO28618) |
抗体コード: | PACO28618 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Nephrin protein (23-257AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO28618 diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO28618 at 1:125, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NPHS1 antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 135, 131 kDa Observed band size: 135 kDa . |
バックグラウンド: | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
シノニム: | Nephrin (Renal glomerulus-specific cell adhesion receptor), NPHS1, NPHN |
UniProt Protein Function: | NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:myosin binding; protein binding Biological Process: excretion; glomerular basement membrane development Disease: Nephrotic Syndrome, Type 1 |
NCBI Summary: | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
UniProt Code: | O60500 |
NCBI GenInfo Identifier: | 20177993 |
NCBI Gene ID: | 4868 |
NCBI Accession: | O60500.1 |
UniProt Secondary Accession: | O60500,A6NDH2, C3RX61, |
UniProt Related Accession: | O60500 |
Molecular Weight: | 130,546 Da |
NCBI Full Name: | Nephrin |
NCBI Synonym Full Names: | NPHS1, nephrin |
NCBI Official Symbol: | NPHS1 |
NCBI Official Synonym Symbols: | CNF; NPHN; nephrin |
NCBI Protein Information: | nephrin |
UniProt Protein Name: | Nephrin |
UniProt Synonym Protein Names: | Renal glomerulus-specific cell adhesion receptor |
Protein Family: | Nephrin |
UniProt Gene Name: | NPHS1 |
UniProt Entry Name: | NPHN_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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