NLRP10 Antibody (PACO57260)
- SKU:
- PACO57260
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NLRP10 Antibody (PACO57260) |
抗体コード: | PACO57260 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human, Rat |
免疫原: | Recombinant Human NACHT, LRR and PYD domains-containing protein 10 protein (589-655AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Rat heart tissue. All lanes: NLRP10 antibody at 4.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76 kDa. Observed band size: 76 kDa. |
![]() | Immunofluorescence staining of Hela cells with PACO57260 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO57260 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID. Displays anti-inflammatory activity. Plays a role in adaptive immunity through control of dendritic cell-mediated transport of antigen to the lymph nodes from peripheral sites. Required for immunity against C.albicans infection. Involved in the innate immune response by contributing to proinflammatory cytokine release in response to invasive bacterial infection. |
シノニム: | NACHT, LRR and PYD domains-containing protein 10 (Nucleotide-binding oligomerization domain protein 8), NLRP10, NALP10 NOD8 PYNOD |
UniProt Protein Function: | NALP10: Inhibits CASP1/caspase-1-dependent IL1B secretion and PYCARD-mediated apoptosis, but not that induced by FAS or BID. Belongs to the NLRP family. |
UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: cytoplasm; extrinsic to plasma membrane Biological Process: defense response to fungus; defense response to Gram-negative bacterium; positive regulation of T-helper 1 type immune response |
NCBI Summary: | Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs to the NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as a multifunctional negative regulator of inflammation and apoptosis. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q86W26 |
NCBI GenInfo Identifier: | 46396380 |
NCBI Gene ID: | 338322 |
NCBI Accession: | Q86W26.1 |
UniProt Secondary Accession: | Q86W26,Q2M3C4, Q6JGT0, |
UniProt Related Accession: | Q86W26 |
Molecular Weight: | 75,032 Da |
NCBI Full Name: | NACHT, LRR and PYD domains-containing protein 10 |
NCBI Synonym Full Names: | NLR family pyrin domain containing 10 |
NCBI Official Symbol: | NLRP10 |
NCBI Official Synonym Symbols: | NOD8; PAN5; PYNOD; NALP10; CLR11.1 |
NCBI Protein Information: | NACHT, LRR and PYD domains-containing protein 10 |
UniProt Protein Name: | NACHT, LRR and PYD domains-containing protein 10 |
UniProt Synonym Protein Names: | Nucleotide-binding oligomerization domain protein 8 |
Protein Family: | NACHT, LRR and PYD domains-containing protein |
UniProt Gene Name: | NLRP10 |
UniProt Entry Name: | NAL10_HUMAN |
Antibodies |
Anti-NLRP10 Antibody (CAB15976) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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