NLN Antibody (PACO60332)
- SKU:
- PACO60332
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NLN Antibody (PACO60332) |
抗体コード: | PACO60332 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Neurolysin, mitochondrial protein (109-241AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate. All lanes: NLN antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa. Observed band size: 81 kDa. |
![]() | Immunofluorescence staining of HepG2 cells with PACO60332 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO60332 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A. |
シノニム: | Neurolysin, mitochondrial (EC 3.4.24.16) (Angiotensin-binding protein) (Microsomal endopeptidase) (MEP) (Mitochondrial oligopeptidase M) (Neurotensin endopeptidase), NLN, AGTBP KIAA1226 |
UniProt Protein Function: | NLN: Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A. Belongs to the peptidase M3 family. |
UniProt Protein Details: | Protein type:EC 3.4.24.16; Mitochondrial; Protease Chromosomal Location of Human Ortholog: 5q12.3 Cellular Component: plasma membrane; mitochondrial intermembrane space Molecular Function:metalloendopeptidase activity; metal ion binding; peptide binding Biological Process: proteolysis |
NCBI Summary: | This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.[provided by RefSeq, Jun 2010] |
UniProt Code: | Q9BYT8 |
NCBI GenInfo Identifier: | 14149738 |
NCBI Gene ID: | 57486 |
NCBI Accession: | NP_065777.1 |
UniProt Secondary Accession: | Q9BYT8,Q9ULJ4, |
UniProt Related Accession: | Q9BYT8 |
Molecular Weight: | 80,652 Da |
NCBI Full Name: | neurolysin, mitochondrial |
NCBI Synonym Full Names: | neurolysin (metallopeptidase M3 family) |
NCBI Official Symbol: | NLN |
NCBI Official Synonym Symbols: | MEP; MOP; AGTBP; EP24.16 |
NCBI Protein Information: | neurolysin, mitochondrial; endopeptidase 24.16; microsomal endopeptidase; neurotensin endopeptidase; angiotensin binding protein; angiotensin-binding protein; mitochondrial oligopeptidase M |
UniProt Protein Name: | Neurolysin, mitochondrial |
UniProt Synonym Protein Names: | Angiotensin-binding protein; Microsomal endopeptidase; MEP; Mitochondrial oligopeptidase M; Neurotensin endopeptidase |
Protein Family: | Neurolysin |
UniProt Gene Name: | NLN |
UniProt Entry Name: | NEUL_HUMAN |