NIT1 Antibody (PACO37246)
- SKU:
- PACO37246
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NIT1 Antibody (PACO37246) |
抗体コード: | PACO37246 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human Deaminated glutathione amidase protein (1-243AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Mouse brain tissue, Rat brain tissue. All lanes: NIT1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36, 32, 41, 35, 27 kDa. Observed band size: 36 kDa. |
![]() | Immunofluorescence staining of HepG2 cells with PACO37246 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO37246 diluted at 1:1200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. |
シノニム: | Deaminated glutathione amidase (dGSH amidase) (EC 3.5.1.-) (Nitrilase homolog 1), NIT1 |
UniProt Protein Function: | NIT1: Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2. Belongs to the UPF0012 family. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 3.5.-.-; Hydrolase Chromosomal Location of Human Ortholog: 1q21-q22 Cellular Component: nucleus Molecular Function:nitrilase activity |
NCBI Summary: | This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
UniProt Code: | Q86X76 |
NCBI GenInfo Identifier: | 51704324 |
NCBI Gene ID: | 4817 |
NCBI Accession: | Q86X76.2 |
UniProt Secondary Accession: | Q86X76,O76091, B1AQP3, D3DVF4, |
UniProt Related Accession: | Q86X76 |
Molecular Weight: | 26,326 Da |
NCBI Full Name: | Nitrilase homolog 1 |
NCBI Synonym Full Names: | nitrilase 1 |
NCBI Official Symbol: | NIT1 |
NCBI Protein Information: | nitrilase homolog 1 |
UniProt Protein Name: | Nitrilase homolog 1 |
Protein Family: | Nitrilase |
UniProt Gene Name: | NIT1 |
UniProt Entry Name: | NIT1_HUMAN |