NEDD8 Antibody (PACO43252)
- SKU:
- PACO43252
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NEDD8 Antibody (PACO43252) |
抗体コード: | PACO43252 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human NEDD8 protein (1-81AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: NEDD8 antibody at 16µg/ml. Lane 1: Mouse heart tissue. Lane 2: Mouse spleen tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 9 kDa. Observed band size: 9 kDa. |
![]() | Immunofluorescent analysis of Hela cells using PACO43252 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | Immunohistochemistry of paraffin-embedded human breast cancer using PACO43252 at dilution of 1:100. |
バックグラウンド: | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. |
シノニム: | NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8), NEDD8 |
UniProt Protein Function: | NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain. |
UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 14q12 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; protein localization; anatomical structure morphogenesis; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance |
UniProt Code: | Q15843 |
NCBI GenInfo Identifier: | 2833270 |
NCBI Gene ID: | 4738 |
NCBI Accession: | Q15843.1 |
UniProt Secondary Accession: | Q15843,Q3SXN8, Q6LES6, |
UniProt Related Accession: | Q15843 |
Molecular Weight: | 81 |
NCBI Full Name: | NEDD8 |
NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 8 |
NCBI Official Symbol: | NEDD8 |
NCBI Official Synonym Symbols: | NEDD-8 |
NCBI Protein Information: | NEDD8; neddylin; ubiquitin-like protein Nedd8 |
UniProt Protein Name: | NEDD8 |
UniProt Synonym Protein Names: | Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 |
Protein Family: | NEDD8 |
UniProt Gene Name: | NEDD8 |
UniProt Entry Name: | NEDD8_HUMAN |
Antibodies | ELISA Kits |
Anti-NEDD8 Antibody (CAB1163) | NEDD8 Colorimetric Cell-Based ELISA Kit |
Anti-NEDD8 Antibody (CAB13520) | |
NEDD8 Antibody (PACO01150) | |
NEDD8 Antibody (PACO10809) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |