NCK1 Antibody (PACO42934)
- SKU:
- PACO42934
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | NCK1 Antibody (PACO42934) |
抗体コード: | PACO42934 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
反応性: | Human, Rat |
免疫原: | Recombinant Human Cytoplasmic protein NCK1 protein (58-377AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: Cytoplasmic protein NCK1 antibody at 4µg/ml + Rat liver tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43, 36 kDa. Observed band size: 43 kDa. |
![]() | Immunohistochemistry of paraffin-embedded human colon cancer using PACO42934 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO42934 at dilution of 1:100. |
バックグラウンド: | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. |
シノニム: | Cytoplasmic protein NCK1 (NCK adaptor protein 1) (Nck-1) (SH2/SH3 adaptor protein NCK-alpha), NCK1, NCK |
UniProt Protein Function: | NCK1: adapter protein containing Src homology 2 and 3 (SH2 and SH3) domains. Transduces signals from tyrosine-phosphorylated growth factor receptors to their cellular substrates. |
UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q21 Cellular Component: endoplasmic reticulum; cytoplasm; plasma membrane; ribosome; protein phosphatase type 1 complex; intercellular junction; vesicle membrane; cytosol; nucleus Molecular Function:protein binding, bridging; protein domain specific binding; protein binding; receptor signaling complex scaffold activity; protein kinase inhibitor activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity; receptor tyrosine kinase binding; receptor binding Biological Process: lamellipodium biogenesis; axon guidance; positive regulation of translation in response to stress; T cell activation; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; response to other organism; T cell receptor signaling pathway; substrate-bound cell migration, cell extension; positive regulation of actin filament polymerization; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway |
NCBI Summary: | The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] |
UniProt Code: | P16333 |
NCBI GenInfo Identifier: | 127962 |
NCBI Gene ID: | 4690 |
NCBI Accession: | P16333.1 |
UniProt Secondary Accession: | P16333,B7Z751, D3DNE3, |
UniProt Related Accession: | P16333 |
Molecular Weight: | 377 |
NCBI Full Name: | Cytoplasmic protein NCK1 |
NCBI Synonym Full Names: | NCK adaptor protein 1 |
NCBI Official Symbol: | NCK1 |
NCBI Official Synonym Symbols: | NCK; nck-1; NCKalpha |
NCBI Protein Information: | cytoplasmic protein NCK1; NCK tyrosine kinase; melanoma NCK protein; SH2/SH3 adaptor protein NCK-alpha; non-catalytic region of tyrosine kinase |
UniProt Protein Name: | Cytoplasmic protein NCK1 |
UniProt Synonym Protein Names: | NCK adaptor protein 1; Nck-1; SH2/SH3 adaptor protein NCK-alpha |
Protein Family: | Nck-associated protein |
UniProt Gene Name: | NCK1 |
UniProt Entry Name: | NCK1_HUMAN |
Antibodies |
Anti-NCK1 Antibody (CAB0999) |
Anti-NCK1 Antibody (CAB13332) |
Anti-Nck Antibody (CAB9129) |
NCK1 Antibody (PACO10755) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |