MUC20 Antibody (PACO57328)
- SKU:
- PACO57328
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | MUC20 Antibody (PACO57328) |
抗体コード: | PACO57328 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Mucin-20 protein (569-657AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: MUC20 antibody at 5.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 72, 69 kDa. Observed band size: 72 kDa. |
![]() | IHC image of PACO57328 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO57328 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. |
シノニム: | Mucin-20 (MUC-20), MUC20, KIAA1359 |
UniProt Protein Function: | MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3q29 Cellular Component: Golgi lumen; microvillus membrane; apical plasma membrane; basal plasma membrane; extracellular region Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization |
NCBI Summary: | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014] |
UniProt Code: | Q8N307 |
NCBI GenInfo Identifier: | 317373415 |
NCBI Gene ID: | 200958 |
NCBI Accession: | Q8N307.3 |
UniProt Secondary Accession: | Q8N307,Q6UX97, Q76I83, Q76I85, Q86ST8, Q8NBY6, Q96KA1 Q9P2I8, |
UniProt Related Accession: | Q8N307 |
Molecular Weight: | 538 |
NCBI Full Name: | Mucin-20 |
NCBI Synonym Full Names: | mucin 20, cell surface associated |
NCBI Official Symbol: | MUC20 |
NCBI Official Synonym Symbols: | MUC-20 |
NCBI Protein Information: | mucin-20; transmembrane mucin MUC20S |
UniProt Protein Name: | Mucin-20 |
Protein Family: | Mucin |
UniProt Gene Name: | MUC20 |
UniProt Entry Name: | MUC20_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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