ME1 Antibody (PACO20002)
- SKU:
- PACO20002
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ME1 Antibody (PACO20002) |
抗体コード: | PACO20002 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
反応性: | Human, Mouse |
免疫原: | Synthetic peptide of human ME1 |
憲法: | Liquid |
ストレージバッファ: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
精製方法: | Antigen affinity purification |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20002(ME1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200). |
![]() | Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane 1-2: 293T cells, A431 cells, Primary antibody: PACO20002(ME1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds. |
![]() | The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO20002(ME1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200). |
バックグラウンド: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid, biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid, cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. |
シノニム: | malic enzyme 1, NADP(+)-dependent, cytosolic |
UniProt Protein Function: | ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting. |
UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.1.1.40; Carbohydrate Metabolism - pyruvate Chromosomal Location of Human Ortholog: 6q12 Cellular Component: mitochondrion; cytosol Molecular Function:malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; ADP binding; NADP binding; NAD binding; oxaloacetate decarboxylase activity Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization |
NCBI Summary: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008] |
UniProt Code: | P48163 |
NCBI GenInfo Identifier: | 1346484 |
NCBI Gene ID: | 4199 |
NCBI Accession: | P48163.1 |
UniProt Secondary Accession: | P48163,Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3 Q9UIY4, B4DZ70, |
UniProt Related Accession: | P48163 |
Molecular Weight: | 572 |
NCBI Full Name: | NADP-dependent malic enzyme |
NCBI Synonym Full Names: | malic enzyme 1, NADP(+)-dependent, cytosolic |
NCBI Official Symbol: | ME1 |
NCBI Official Synonym Symbols: | MES; HUMNDME |
NCBI Protein Information: | NADP-dependent malic enzyme; NADP-ME; malate dehydrogenase; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; pyruvic-malic carboxylase |
UniProt Protein Name: | NADP-dependent malic enzyme |
UniProt Synonym Protein Names: | Malic enzyme 1 |
Protein Family: | NADP-dependent malic enzyme |
UniProt Gene Name: | ME1 |
UniProt Entry Name: | MAOX_HUMAN |