MCC Antibody (PACO58821)
- SKU:
- PACO58821
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | MCC Antibody (PACO58821) |
抗体コード: | PACO58821 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Colorectal mutant cancer protein (479-611AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO58821 diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO58821 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO58821 diluted at 1:500 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. Represses the beta-catenin pathway (canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin (CTNB1) transcriptional activity. |
シノニム: | Colorectal mutant cancer protein (Protein MCC), MCC |
UniProt Protein Function: | MCC: Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b- catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. Belongs to the MCC family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Tumor suppressor; Cell cycle regulation Chromosomal Location of Human Ortholog: 5q21 Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; receptor activity Biological Process: establishment of protein localization; negative regulation of epithelial cell proliferation; signal transduction |
NCBI Summary: | This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P23508 |
NCBI GenInfo Identifier: | 317373352 |
NCBI Gene ID: | 4163 |
NCBI Accession: | P23508.2 |
UniProt Secondary Accession: | P23508,Q6ZR04, D3DT05, |
UniProt Related Accession: | P23508 |
Molecular Weight: | 113,279 Da |
NCBI Full Name: | Colorectal mutant cancer protein |
NCBI Synonym Full Names: | mutated in colorectal cancers |
NCBI Official Symbol: | MCC |
NCBI Official Synonym Symbols: | MCC1 |
NCBI Protein Information: | colorectal mutant cancer protein |
UniProt Protein Name: | Colorectal mutant cancer protein |
Protein Family: | Colorectal mutant cancer protein |
UniProt Gene Name: | MCC |
UniProt Entry Name: | CRCM_HUMAN |