KPNA1 Antibody (PACO27841)
- SKU:
- PACO27841
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | KPNA1 Antibody (PACO27841) |
抗体コード: | PACO27841 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Importin subunit alpha-5 protein (8-538AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO27841 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human lung cancer using PACO27841 at dilution of 1:100. |
バックグラウンド: | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-β and the three components separate and importin-α and -β are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. |
シノニム: | Importin subunit alpha-5 (Karyopherin subunit alpha-1) (Nucleoprotein interactor 1) (NPI-1) (RAG cohort protein 2) (SRP1-beta) [Cleaved into: Importin subunit alpha-5, N-terminally processed], KPNA1, RCH2 |
UniProt Protein Function: | KPNA1: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Belongs to the importin alpha family. |
UniProt Protein Details: | Protein type:Nuclear import; Karyopherin; Adaptor/scaffold Chromosomal Location of Human Ortholog: 3q21 Cellular Component: nucleoplasm; dendrite; cytoplasm; nuclear pore; cytosol; nucleus Molecular Function:protein binding; nuclear localization sequence binding; protein transporter activity Biological Process: viral reproduction; positive regulation of protein import into nucleus; apoptosis; regulation of DNA recombination; cytokine and chemokine mediated signaling pathway; DNA fragmentation during apoptosis; NLS-bearing substrate import into nucleus; viral infectious cycle; cell structure disassembly during apoptosis |
NCBI Summary: | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] |
UniProt Code: | P52294 |
NCBI GenInfo Identifier: | 296439328 |
NCBI Gene ID: | 3836 |
NCBI Accession: | P52294.3 |
UniProt Related Accession: | P52294 |
Molecular Weight: | |
NCBI Full Name: | Importin subunit alpha-5 |
NCBI Synonym Full Names: | karyopherin subunit alpha 1 |
NCBI Official Symbol: | KPNA1 |
NCBI Official Synonym Symbols: | RCH2; SRP1; IPOA5; NPI-1 |
NCBI Protein Information: | importin subunit alpha-5 |
UniProt Protein Name: | Importin subunit alpha-5 |
UniProt Synonym Protein Names: | Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-betaImportin subunit alpha-5, N-terminally processed |
Protein Family: | Integral inner nuclear membrane protein |
UniProt Gene Name: | KPNA1 |
UniProt Entry Name: | IMA5_HUMAN |