KLK1 Antibody (PACO27813)
- SKU:
- PACO27813
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | KLK1 Antibody (PACO27813) |
抗体コード: | PACO27813 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Kallikrein-1 protein (25-262AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO27813 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO27813 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO27813 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. |
シノニム: | Kallikrein-1 (EC 3.4.21.35) (Kidney/pancreas/salivary gland kallikrein) (Tissue kallikrein), KLK1 |
UniProt Protein Function: | KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protease; EC 3.4.21.35 Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleus Molecular Function:serine-type endopeptidase activity Biological Process: proteolysis Disease: Kallikrein, Decreased Urinary Activity Of |
NCBI Summary: | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008] |
UniProt Code: | P06870 |
NCBI GenInfo Identifier: | 269849612 |
NCBI Gene ID: | 3816 |
NCBI Accession: | P06870.2 |
UniProt Secondary Accession: | P06870,Q66US9, Q86U61, Q8TCV8, Q9BS53, Q9NQU4, Q9UD19 Q9UMJ1, |
UniProt Related Accession: | P06870 |
Molecular Weight: | 23,835 Da |
NCBI Full Name: | Kallikrein-1 |
NCBI Synonym Full Names: | kallikrein 1 |
NCBI Official Symbol: | KLK1 |
NCBI Official Synonym Symbols: | hK1; KLKR; Klk6 |
NCBI Protein Information: | kallikrein-1; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; kidney/pancreas/salivary gland kallikrein |
UniProt Protein Name: | Kallikrein-1 |
UniProt Synonym Protein Names: | Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein |
Protein Family: | Kallikrein 1-related peptidase |
UniProt Gene Name: | KLK1 |
UniProt Entry Name: | KLK1_HUMAN |
Antibodies | ELISA Kits |
Anti-KLK1 Antibody (CAB1807) | Human Kallikrein-1 (KLK1) ELISA Kit |
KLK1 Antibody (PACO02125) | |
KLK1 Antibody (PACO10142) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |