KIFAP3 Antibody (PACO56864)
- SKU:
- PACO56864
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | KIFAP3 Antibody (PACO56864) |
抗体コード: | PACO56864 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Kinesin-associated protein 3 protein (660-792AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunofluorescence staining of HepG2 cells with PACO56864 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO56864 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | IHC image of PACO56864 diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity). |
シノニム: | Kinesin-associated protein 3 (KAP-3) (KAP3) (Smg GDS-associated protein), KIFAP3, KIF3AP SMAP |
UniProt Protein Function: | KIFAP3: Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex. |
UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 1q24.2 Cellular Component: microtubule cytoskeleton; Golgi apparatus; centrosome; condensed nuclear chromosome; endoplasmic reticulum; spindle microtubule; kinesin II complex; axoneme; cytosol; photoreceptor connecting cilium Molecular Function:protein binding; kinesin binding Biological Process: negative regulation of cell proliferation; protein localization; positive regulation of calcium-dependent cell-cell adhesion; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein complex assembly; blood coagulation; signal transduction; microtubule-based movement; microtubule-based process; negative regulation of apoptosis |
NCBI Summary: | The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
UniProt Code: | Q92845 |
NCBI GenInfo Identifier: | 73920216 |
NCBI Gene ID: | 22920 |
NCBI Accession: | Q92845.2 |
UniProt Secondary Accession: | Q92845,Q8NHU7, Q9H416, B1AKU4, B1AKU5, B2RDL1, B7Z8A3 F5H591, |
UniProt Related Accession: | Q92845 |
Molecular Weight: | 792 |
NCBI Full Name: | Kinesin-associated protein 3 |
NCBI Synonym Full Names: | kinesin-associated protein 3 |
NCBI Official Symbol: | KIFAP3 |
NCBI Official Synonym Symbols: | FLA3; KAP3; SMAP; KAP-1; KAP-3; Smg-GDS; dJ190I16.1 |
NCBI Protein Information: | kinesin-associated protein 3; smg GDS-associated protein; small G protein GDP dissociation stimulator |
UniProt Protein Name: | Kinesin-associated protein 3 |
UniProt Synonym Protein Names: | Smg GDS-associated protein |
Protein Family: | Kinesin-associated protein |
UniProt Gene Name: | KIFAP3 |
UniProt Entry Name: | KIFA3_HUMAN |