Keap1 Antibody (PACO27766)
- SKU:
- PACO27766
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | Keap1 Antibody (PACO27766) |
抗体コード: | PACO27766 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000 |
反応性: | Mouse, Human |
免疫原: | Recombinant Mouse Kelch-like ECH-associated protein 1 protein (1-624AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot All lanes: Keap1 antibody at 9µg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa . |
バックグラウンド: | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity). |
シノニム: | Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2), Keap1, Inrf2 Kiaa0132 |
UniProt Protein Function: | KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system; Endoplasmic reticulum; Transcription regulationCellular Component: nucleoplasm; cell-cell adherens junction; focal adhesion; endoplasmic reticulum; cytoplasm; microtubule organizing center; midbody; nucleusMolecular Function: protein binding; transcription factor bindingBiological Process: cytoplasmic sequestering of transcription factor; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of epidermal cell differentiation; protein oligomerization |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | Q9Z2X8 |
NCBI GenInfo Identifier: | 160333659 |
NCBI Gene ID: | 50868 |
NCBI Accession: | NP_001103775.1 |
UniProt Secondary Accession: | Q9Z2X8,Q3U243, Q3U8N7, Q547S3, Q6ZQI6, Q8BQY3 |
UniProt Related Accession: | Q9Z2X8 |
Molecular Weight: | 69,553 Da |
NCBI Full Name: | kelch-like ECH-associated protein 1 |
NCBI Synonym Full Names: | kelch-like ECH-associated protein 1 |
NCBI Official Symbol: | Keap1 |
NCBI Official Synonym Symbols: | INRF2; mKIAA0132 |
NCBI Protein Information: | kelch-like ECH-associated protein 1; ring canal protein; NRF2 cytosolic inhibitor; cytosolic inhibitor of Nrf2 |
UniProt Protein Name: | Kelch-like ECH-associated protein 1 |
UniProt Synonym Protein Names: | Cytosolic inhibitor of Nrf2; INrf2 |
Protein Family: | Kelch-like ECH-associated protein |
UniProt Gene Name: | Keap1 |
UniProt Entry Name: | KEAP1_MOUSE |