KDM1A Antibody (PACO46470)
- SKU:
- PACO46470
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | KDM1A Antibody (PACO46470) |
抗体コード: | PACO46470 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Lysine-specific histone demethylase 1A protein (100-150AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Mouse liver tissue. All lanes: KDM1A antibody at 2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 96 kDa. Observed band size: 93 kDa.. | |
Immunofluorescent analysis of Hela cells using PACO46470 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human testis tissue using PACO46470 at dilution of 1:100. |
バックグラウンド: | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. |
シノニム: | Lysine-specific histone demethylase 1A (EC 1) (BRAF35-HDAC complex protein BHC110) (Flavin-containing amine oxidase domain-containing protein 2), KDM1A, AOF2 KDM1 KIAA0601 LSD1 |
UniProt Code: | O60341 |
NCBI GenInfo Identifier: | 58761544 |
NCBI Gene ID: | 23028 |
NCBI Accession: | NP_001009999.1 |
UniProt Secondary Accession: | O60341,Q5TH94, Q5TH95, Q86VT7, Q8IXK4, Q8NDP6, Q8TAZ3 Q96AW4, A8MWP9, |
UniProt Related Accession: | O60341 |
Molecular Weight: | 852 |
NCBI Full Name: | lysine-specific histone demethylase 1A isoform a |
NCBI Synonym Full Names: | lysine (K)-specific demethylase 1A |
NCBI Official Symbol: | KDM1A |
NCBI Official Synonym Symbols: | AOF2; KDM1; LSD1; BHC110 |
NCBI Protein Information: | lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-con |
UniProt Protein Name: | Lysine-specific histone demethylase 1A |
UniProt Synonym Protein Names: | BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2 |
Protein Family: | Lysine-specific histone demethylase |
UniProt Gene Name: | KDM1A |
UniProt Entry Name: | KDM1A_HUMAN |