IRF2BP1 Antibody (PACO56018)
- SKU:
- PACO56018
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | IRF2BP1 Antibody (PACO56018) |
抗体コード: | PACO56018 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Interferon regulatory factor 2-binding protein 1 protein (340-499AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO56018 diluted at 1:100 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO56018 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation. |
シノニム: | Interferon regulatory factor 2-binding protein 1 (IRF-2-binding protein 1) (IRF-2BP1) (Probable E3 ubiquitin-protein ligase IRF2BP1) (EC 2.3.2.27) (Probable RING-type E3 ubiquitin transferase IRF2BP1), IRF2BP1 |
UniProt Protein Function: | IRF2BP1: Acts as a transcriptional corepressor in a IRF2- dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation. Belongs to the IRF2BP family. |
UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; EC 6.3.2.- Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: nucleoplasm; intracellular membrane-bound organelle Molecular Function:protein binding; metal ion binding; ligase activity Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent |
UniProt Code: | Q8IU81 |
NCBI GenInfo Identifier: | 74727955 |
NCBI Gene ID: | 26145 |
NCBI Accession: | Q8IU81.1 |
UniProt Secondary Accession: | Q8IU81,Q53EL7, Q6DC95, Q9BRZ9, Q9Y4P4, |
UniProt Related Accession: | Q8IU81 |
Molecular Weight: | 584 |
NCBI Full Name: | Interferon regulatory factor 2-binding protein 1 |
NCBI Synonym Full Names: | interferon regulatory factor 2 binding protein 1 |
NCBI Official Symbol: | IRF2BP1 |
NCBI Protein Information: | interferon regulatory factor 2-binding protein 1; IRF-2-binding protein 1; probable E3 ubiquitin-protein ligase IRF2BP1 |
UniProt Protein Name: | Interferon regulatory factor 2-binding protein 1 |
UniProt Synonym Protein Names: | Probable E3 ubiquitin-protein ligase IRF2BP1 (EC:6.3.2.-) |
Protein Family: | Interferon regulatory factor 2-binding protein |
UniProt Gene Name: | IRF2BP1 |
UniProt Entry Name: | I2BP1_HUMAN |