IDH3B Antibody (PACO31988)
- SKU:
- PACO31988
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | IDH3B Antibody (PACO31988) |
抗体コード: | PACO31988 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial protein (35-385AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: IDH3B antibody at 2µg/ml Lane 1: Hela whole cell lysate Lane 2: Mouse kidney tissue Lane 3: Mouse gonadal tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 42, 26 kDa Observed band size: 43 kDa . |
シノニム: | Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (Isocitric dehydrogenase subunit beta) (NAD(+)-specific ICDH subunit beta), IDH3B |
UniProt Protein Function: | IDH3B: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.1.1.41; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle Chromosomal Location of Human Ortholog: 20p13 Cellular Component: mitochondrial matrix; mitochondrion; nucleus Molecular Function:electron carrier activity Biological Process: tricarboxylic acid cycle Disease: Retinitis Pigmentosa 46 |
NCBI Summary: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | O43837 |
NCBI GenInfo Identifier: | 146345439 |
NCBI Gene ID: | 3420 |
NCBI Accession: | O43837.2 |
UniProt Secondary Accession: | O43837,O95106, Q5JXS8, Q9NQ06, Q9NQ07, Q9NUZ0, Q9UEX0 Q9UG99, B2RDR1, D3DVX2, D3DVX3, |
UniProt Related Accession: | O43837 |
Molecular Weight: | 25,555 Da |
NCBI Full Name: | Isocitrate dehydrogenase |
NCBI Synonym Full Names: | isocitrate dehydrogenase 3 (NAD(+)) beta |
NCBI Official Symbol: | IDH3B |
NCBI Official Synonym Symbols: | RP46 |
NCBI Protein Information: | isocitrate dehydrogenase [NAD] subunit beta, mitochondrial |
UniProt Protein Name: | Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial |
UniProt Synonym Protein Names: | Isocitric dehydrogenase subunit beta; NAD(+)-specific ICDH subunit beta |
Protein Family: | Isocitrate dehydrogenase |
UniProt Gene Name: | IDH3B |
UniProt Entry Name: | IDH3B_HUMAN |
Antibodies |
Anti-IDH3B Antibody (CAB13742) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |