人間 Thrombospondin-1 (THBS1) ELISA Kit

商品名:	Human Thrombospondin-1 (THBS1) ELISA Kit
製品コード:	HUEB0203
エイリアス:	Thrombospondin-1, THBS1, TSP, TSP1
Uniprot:	P07996
反応性:	Human
範囲:	0.156-10 ng/mL
検出方法:	Sandwich
サイズ:	96 Assay
保管所:	Please see kit components below for exact storage details
ノート:	For research use only

UniProt Protein Function:	THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Inhibitor Chromosomal Location of Human Ortholog: 15q15 Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum; endoplasmic reticulum lumen; fibrinogen complex; extracellular region; secretory granule; external side of plasma membrane Molecular Function:heparin binding; identical protein binding; laminin binding; calcium ion binding; integrin binding; protein binding; proteoglycan binding; fibroblast growth factor binding; transforming growth factor beta binding; phosphatidylserine binding; fibronectin binding; low-density lipoprotein binding; glycoprotein binding Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell adhesion; cell cycle arrest; positive regulation of macrophage activation; response to drug; platelet activation; negative regulation of interleukin-12 production; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; response to testosterone stimulus; negative regulation of cell-matrix adhesion; negative regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of angiogenesis; response to mechanical stimulus; negative regulation of endothelial cell proliferation; peptide cross-linking; regulation of cGMP metabolic process; response to calcium ion; response to progesterone stimulus; negative regulation of apoptosis; positive regulation of blood coagulation; positive regulation of translation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; platelet degranulation; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of transforming growth factor-beta1 production; protein amino acid O-linked glycosylation; cell migration; chronic inflammatory response; negative regulation of focal adhesion formation; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; post-translational protein modification; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; cellular protein metabolic process; negative regulation of dendritic cell antigen processing and presentation; response to hypoxia; immune response; sprouting angiogenesis; blood coagulation; positive regulation of phosphorylation; positive regulation of cell migration
NCBI Summary:	The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Code:	P07996
NCBI GenInfo Identifier:	117949802
NCBI Gene ID:	7057
NCBI Accession:	P07996.2
UniProt Secondary Accession:	P07996,Q15667, Q59E99, A8K6H4, B4E3J7, B9EGH6,
UniProt Related Accession:	P07996
Molecular Weight:	120,148 Da
NCBI Full Name:	Thrombospondin-1
NCBI Synonym Full Names:	thrombospondin 1
NCBI Official Symbol:	THBS1
NCBI Official Synonym Symbols:	TSP; THBS; TSP1; TSP-1; THBS-1
NCBI Protein Information:	thrombospondin-1; thrombospondin-1p180
UniProt Protein Name:	Thrombospondin-1
Protein Family:	Thrombospondin
UniProt Gene Name:	THBS1
UniProt Entry Name:	TSP1_HUMAN

成分	額	保管所
ELISA Microplate (Dismountable)	8×12 strips	-20°C
Lyophilized Standard	2	-20°C
Sample Diluent	20ml	-20°C
Assay Diluent A	10mL	-20°C
Assay Diluent B	10mL	-20°C
Detection Reagent A	120µL	-20°C
Detection Reagent B	120µL	-20°C
Wash Buffer	30mL	4°C
Substrate	10mL	4°C
Stop Solution	10mL	4°C
Plate Sealer	5	-

その他の必要な材料と設備：

Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
Incubator
Deionized or distilled water
Absorbent paper
Buffer resevoir

*ノート: プロトコルは、各バッチ/ロットに固有です。正しい手順については、キットに含まれているプロトコルに従ってください。

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

ステップ	プロトコル
1.	Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2.	Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3.	Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4.	Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5.	Repeat the wash process for five times as conducted in step 3.
6.	Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7.	Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8.	Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9.	After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

ELISAアッセイを実施する場合、可能な限り最良の結果を達成するためにサンプルを準備することが重要です。以下に、さまざまなサンプルタイプのサンプルを準備するための手順のリストを示します。

サンプルタイプ:	プロトコル:
血清:	If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
プラズマ:	Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
尿および脳脊髄液:	Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
細胞培養上清:	Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
細胞溶解物:	Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
組織ホモジネート:	The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
組織溶解物:	Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
母乳:	Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

ELISA	Antibodies
Human TSP-1 (Thrombospondin-1) CLIA Kit (HUES00905)	Anti-THBS1 Antibody (CAB16985)
	Anti-THBS1 Antibody (CAB2125)
	TRNAU1AP Antibody (PACO13650)
	CALCA Antibody (PACO19218)
	THBS1 Antibody (PACO19219)