HRH4 Antibody (PACO56218)
- SKU:
- PACO56218
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | HRH4 Antibody (PACO56218) |
抗体コード: | PACO56218 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Histamine H4 receptor protein (204-292AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Mouse liver tissue. All lanes: HRH4 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45, 35 kDa. Observed band size: 45 kDa. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO56218 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO56218 diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist). |
シノニム: | Histamine H4 receptor (H4R) (HH4R) (AXOR35) (G-protein coupled receptor 105) (GPRv53) (Pfi-013) (SP9144), HRH4, GPCR105 |
UniProt Protein Function: | H4R: The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR Chromosomal Location of Human Ortholog: 18q11.2 Cellular Component: plasma membrane; synapse Molecular Function:G-protein coupled acetylcholine receptor activity Biological Process: inflammatory response; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; synaptic transmission, cholinergic |
NCBI Summary: | Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by a family of histamine receptors, which are a subset of the G-protein coupled receptor superfamily. This gene encodes a histamine receptor that is predominantly expressed in haematopoietic cells. The protein is thought to play a role in inflammation and allergy reponses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
UniProt Code: | Q9H3N8 |
NCBI GenInfo Identifier: | 14194819 |
NCBI Gene ID: | 59340 |
NCBI Accession: | Q9H3N8.2 |
UniProt Secondary Accession: | Q9H3N8,Q4G0I6, Q9GZQ0, B0YJ19, B2KJ48, |
UniProt Related Accession: | Q9H3N8 |
Molecular Weight: | 34,497 Da |
NCBI Full Name: | Histamine H4 receptor |
NCBI Synonym Full Names: | histamine receptor H4 |
NCBI Official Symbol: | HRH4 |
NCBI Official Synonym Symbols: | H4; H4R; BG26; HH4R; AXOR35; GPRv53; GPCR105 |
NCBI Protein Information: | histamine H4 receptor |
UniProt Protein Name: | Histamine H4 receptor |
UniProt Synonym Protein Names: | AXOR35; G-protein coupled receptor 105; GPRv53; Pfi-013; SP9144 |
Protein Family: | Histamine H4 receptor |
UniProt Gene Name: | HRH4 |
UniProt Entry Name: | HRH4_HUMAN |