HLTF Antibody (PACO54810)
- SKU:
- PACO54810
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | HLTF Antibody (PACO54810) |
抗体コード: | PACO54810 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IP |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
反応性: | Human |
免疫原: | Recombinant Human Helicase-like transcription factor protein (332-476AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate. All lanes: HLTF antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114, 100 kDa. Observed band size: 114 kDa. |
![]() | Immunoprecipitating HLTF in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PACO54810 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000). Lane 2: PACO54810 (6µg) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20µg). |
![]() | Immunohistochemistry of paraffin-embedded human testis tissue using PACO54810 at dilution of 1:100. |
バックグラウンド: | Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. |
シノニム: | Helicase-like transcription factor (EC 2.3.2.27) (EC 3.6.4) (DNA-binding protein/plasminogen activator inhibitor 1 regulator) (HIP116) (RING finger protein 80) (RING-type E3 ubiquitin transferase HLTF) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3) (Sucrose nonfermenting protein 2-like 3), HLTF, HIP116A RNF80 SMARCA3 SNF2L3 ZBU1 |
UniProt Protein Function: | SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described. |
UniProt Protein Details: | Protein type:Ligase; EC 3.6.1.-; Nucleolus; EC 3.6.4.-; EC 6.3.2.-; Ubiquitin conjugating system; Helicase; EC 6.3.2.19; DNA-binding; Ubiquitin ligase Chromosomal Location of Human Ortholog: 3q25.1-q26.1 Cellular Component: membrane; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding; ubiquitin protein ligase binding Biological Process: regulation of transcription, DNA-dependent |
NCBI Summary: | This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q14527 |
NCBI GenInfo Identifier: | 60390864 |
NCBI Gene ID: | 6596 |
NCBI Accession: | Q14527.2 |
UniProt Secondary Accession: | Q14527,Q14536, Q16051, Q7KYJ6, Q86YA5, Q92652, Q96KM9 D3DNH3, |
UniProt Related Accession: | Q14527 |
Molecular Weight: | 99,919 Da |
NCBI Full Name: | Helicase-like transcription factor |
NCBI Synonym Full Names: | helicase like transcription factor |
NCBI Official Symbol: | HLTF |
NCBI Official Synonym Symbols: | ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3 |
NCBI Protein Information: | helicase-like transcription factor |
UniProt Protein Name: | Helicase-like transcription factor |
UniProt Synonym Protein Names: | DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; RING finger protein 80; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; Sucrose nonfermenting protein 2-like 3 |
Protein Family: | Helicase-like transcription factor |
UniProt Gene Name: | HLTF |
UniProt Entry Name: | HLTF_HUMAN |