HIST1H2BB (Ab-16) Antibody (PACO56508)
- SKU:
- PACO56508
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Application:
- ChIP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | HIST1H2BB (Ab-16) Antibody (PACO56508) |
抗体コード: | PACO56508 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IF, ChIP |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:200-1:2000, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Peptide sequence around site of Lys (16) derived from Human Histone H2B type 1-B |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela cell acid extracts, Jurkat cell acid extracts, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue. All lanes: HIST1H2BB antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa. | |
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2BB (PACO56508) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter. | |
Immunofluorescent analysis of Hela cells using PACO56508 at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG. |
バックグラウンド: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
シノニム: | Histone H2B type 1-B (Histone H2B.1) (Histone H2B.f) (H2B/f), HIST1H2BB, H2BFF |
UniProt Protein Function: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
UniProt Code: | P33778 |
NCBI GenInfo Identifier: | 462236 |
NCBI Gene ID: | 3018 |
NCBI Accession: | P33778.2 |
UniProt Secondary Accession: | P33778,Q4KN36, |
UniProt Related Accession: | P33778 |
Molecular Weight: | 13,950 Da |
NCBI Full Name: | Histone H2B type 1-B |
NCBI Synonym Full Names: | histone cluster 1 H2B family member b |
NCBI Official Symbol: | HIST1H2BB |
NCBI Official Synonym Symbols: | H2B.1; H2B/f; H2BFF |
NCBI Protein Information: | histone H2B type 1-B |
UniProt Protein Name: | Histone H2B type 1-B |
UniProt Synonym Protein Names: | Histone H2B.1; Histone H2B.f; H2B/f |
Protein Family: | Histone |
UniProt Gene Name: | HIST1H2BB |
Antibodies | ELISA Kits |
HIST1H2BB Antibody (PACO04026) | Human HIST1H2BB (Histone H2B type 1-B) ELISA Kit (HUFI04761) |
Histone H2B Colorimetric Cell-Based ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |