GIT1 Antibody (PACO51550)
- SKU:
- PACO51550
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | GIT1 Antibody (PACO51550) |
抗体コード: | PACO51550 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human ARF GTPase-activating protein GIT1 protein (470-642AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Mouse brain tissue. All lanes: GIT1 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 20, 86 kDa. Observed band size: 85 kDa.. |
![]() | Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO51550 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human thyroid tissue using PACO51550 at dilution of 1:100. |
バックグラウンド: | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity). |
シノニム: | ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1), GIT1 |
UniProt Protein Function: | GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs). |
UniProt Protein Details: | Protein type:GAPs, ARF; Motility/polarity/chemotaxis; GAPs Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane Molecular Function:protein binding Biological Process: ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway |
UniProt Code: | Q9Y2X7 |
NCBI GenInfo Identifier: | 45645212 |
NCBI Gene ID: | 28964 |
NCBI Accession: | Q9Y2X7.2 |
UniProt Secondary Accession: | Q9Y2X7,Q86SS0, Q9BRJ4, B4DGU9, B4DSV3, |
UniProt Related Accession: | Q9Y2X7 |
Molecular Weight: | 85,446 Da |
NCBI Full Name: | ARF GTPase-activating protein GIT1 |
NCBI Synonym Full Names: | GIT ArfGAP 1 |
NCBI Official Symbol: | GIT1 |
NCBI Protein Information: | ARF GTPase-activating protein GIT1 |
UniProt Protein Name: | ARF GTPase-activating protein GIT1 |
UniProt Synonym Protein Names: | Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 |
Protein Family: | ARF GTPase-activating protein |
UniProt Gene Name: | GIT1 |
UniProt Entry Name: | GIT1_HUMAN |