FREM2 Antibody (PACO57196)
- SKU:
- PACO57196
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | FREM2 Antibody (PACO57196) |
抗体コード: | PACO57196 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human FRAS1-related extracellular matrix protein 2 protein (1986-2127AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of MCF-7 cells with PACO57196 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO57196 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion. |
シノニム: | FRAS1-related extracellular matrix protein 2 (ECM3 homolog), FREM2 |
UniProt Protein Function: | FREM2: Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion. Defects in FREM2 are a cause of Fraser syndrome (FRASS). Fraser syndrome is a multisystem malformation usually comprising cryptophthalmos, cutaneous syndactyly, ear abnormalities, renal agenesis and congenital heart defects. Belongs to the FRAS1 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Extracellular matrix Chromosomal Location of Human Ortholog: 13q13.3 Cellular Component: plasma membrane; integral to membrane; basement membrane Molecular Function:calcium ion binding Biological Process: morphogenesis of an epithelium; cell communication; homophilic cell adhesion; inner ear development Disease: Fraser Syndrome |
NCBI Summary: | This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014] |
UniProt Code: | Q5SZK8 |
NCBI GenInfo Identifier: | 79749430 |
NCBI Gene ID: | 341640 |
NCBI Accession: | NP_997244 |
UniProt Secondary Accession: | Q5SZK8,Q4QQG1, Q5H9N8, Q5T6Q1, Q6N057, Q6ZSB4, Q7Z305 Q7Z341, |
UniProt Related Accession: | Q5SZK8 |
Molecular Weight: | 295,845 Da |
NCBI Full Name: | FRAS1-related extracellular matrix protein 2 |
NCBI Synonym Full Names: | FRAS1 related extracellular matrix protein 2 |
NCBI Official Symbol: | FREM2 |
NCBI Protein Information: | FRAS1-related extracellular matrix protein 2; ECM3 homolog |
UniProt Protein Name: | FRAS1-related extracellular matrix protein 2 |
UniProt Synonym Protein Names: | ECM3 homolog |
Protein Family: | FRAS1-related extracellular matrix protein |
UniProt Gene Name: | FREM2 |
UniProt Entry Name: | FREM2_HUMAN |
Antibodies |
Anti-FREM2 Antibody (CAB15980) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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