FBXO10 Antibody (PACO59541)
- SKU:
- PACO59541
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | FBXO10 Antibody (PACO59541) |
抗体コード: | PACO59541 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human F-box only protein 10 protein (248-400AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO59541 diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO59541 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO59541 diluted at 1:500 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. The SCF(FBXO10) complex mediates ubiquitination and degradation of BCL2, an antiapoptotic protein, thereby playing a role in apoptosis by controlling the stability of BCL2. |
シノニム: | F-box only protein 10, FBXO10, FBX10 PRMT11 |
UniProt Protein Function: | PRMT11: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. |
UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 9p13.2 Cellular Component: cytoplasm; ubiquitin ligase complex Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: regulation of apoptosis; apoptosis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination |
NCBI Summary: | Members of the F-box protein family, such as FBXO10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
UniProt Code: | Q9UK96 |
NCBI GenInfo Identifier: | 124244083 |
NCBI Gene ID: | 26267 |
NCBI Accession: | NP_036298.2 |
UniProt Secondary Accession: | Q9UK96,Q08AL3, Q5JRT8, Q9UKC3, |
UniProt Related Accession: | Q9UK96 |
Molecular Weight: | 105,195 Da |
NCBI Full Name: | F-box only protein 10 |
NCBI Synonym Full Names: | F-box protein 10 |
NCBI Official Symbol: | FBXO10 |
NCBI Official Synonym Symbols: | FBX10; PRMT11 |
NCBI Protein Information: | F-box only protein 10; F-box protein Fbx10 |
UniProt Protein Name: | F-box only protein 10 |
Protein Family: | F-box only protein |
UniProt Gene Name: | FBXO10 |
UniProt Entry Name: | FBX10_HUMAN |
Antibodies |
Anti-FBXO10 Antibody (CAB14871) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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