EPHA8 Antibody (PACO63647)
- SKU:
- PACO63647
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | EPHA8 Antibody (PACO63647) |
抗体コード: | PACO63647 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Ephrin type-A receptor 8 protein (45-249AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of SH-SY5Y cells with PACO63647 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system plays also a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth (By similarity). |
シノニム: | Ephrin type-A receptor 8 (EC 2.7.10.1) (EPH- and ELK-related kinase) (EPH-like kinase 3) (EK3) (hEK3) (Tyrosine-protein kinase receptor EEK), EPHA8, EEK HEK3 KIAA1459 |
UniProt Protein Function: | EphA8: a receptor tyrosine kinase of the Eph family. A receptor for members of the ephrin-A family: ephrin A2, A3 and A5. Plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Contains 1 sterile alpha motif (SAM) domain and 2 fibronectin type III domains. |
UniProt Protein Details: | Protein type:EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group Chromosomal Location of Human Ortholog: 1p36.12 Cellular Component: integral to plasma membrane; neuron projection; plasma membrane Molecular Function:GPI-linked ephrin receptor activity Biological Process: axon guidance; ephrin receptor signaling pathway; neurite development; neuron remodeling; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; protein amino acid autophosphorylation; regulation of cell adhesion; regulation of cell adhesion mediated by integrin; substrate-bound cell migration |
NCBI Summary: | This gene encodes a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The protein encoded by this gene functions as a receptor for ephrin A2, A3 and A5 and plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. [provided by RefSeq, Jul 2008] |
UniProt Code: | P29322 |
NCBI GenInfo Identifier: | 19857975 |
NCBI Gene ID: | 2046 |
NCBI Accession: | P29322.2 |
UniProt Secondary Accession: | P29322,Q6IN80, Q8IUX6, Q9NUA9, Q9P269, |
UniProt Related Accession: | P29322 |
Molecular Weight: | 111kDa |
NCBI Full Name: | Ephrin type-A receptor 8 |
NCBI Synonym Full Names: | EPH receptor A8 |
NCBI Official Symbol: | EPHA8 |
NCBI Official Synonym Symbols: | EEK; EK3; HEK3 |
NCBI Protein Information: | ephrin type-A receptor 8 |
UniProt Protein Name: | Ephrin type-A receptor 8 |
UniProt Synonym Protein Names: | EPH- and ELK-related kinase; EPH-like kinase 3; EK3; hEK3; Tyrosine-protein kinase receptor EEK |
Protein Family: | Ephrin type-A receptor |
UniProt Gene Name: | EPHA8 |