EPHA4 Antibody (PACO48890)
- SKU:
- PACO48890
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | EPHA4 Antibody (PACO48890) |
抗体コード: | PACO48890 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Ephrin type-A receptor 4 protein (345-545AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunohistochemistry of paraffin-embedded human spleen tissue using PACO48890 at dilution of 1:100. |
![]() | Immunofluorescent analysis of Hela cells using PACO48890 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO48890 at dilution of 1:100. |
バックグラウンド: | Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. |
シノニム: | Ephrin type-A receptor 4 (EC 2.7.10.1) (EPH-like kinase 8) (EK8) (hEK8) (Tyrosine-protein kinase TYRO1) (Tyrosine-protein kinase receptor SEK), EPHA4, HEK8 SEK TYRO1 |
UniProt Protein Function: | EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. |
UniProt Protein Details: | Protein type:EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; TK group; Eph family Chromosomal Location of Human Ortholog: 2q36.1 Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; axon; early endosome membrane; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium Molecular Function:identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; motor axon guidance; regulation of astrocyte differentiation; positive regulation of dendrite morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior; ephrin receptor signaling pathway; corticospinal tract morphogenesis; cell adhesion |
NCBI Summary: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008] |
UniProt Code: | P54764 |
NCBI GenInfo Identifier: | 4758280 |
NCBI Gene ID: | 2043 |
NCBI Accession: | NP_004429.1 |
UniProt Secondary Accession: | P54764,Q2M380, |
UniProt Related Accession: | P54764 |
Molecular Weight: | 109,860 Da |
NCBI Full Name: | ephrin type-A receptor 4 |
NCBI Synonym Full Names: | EPH receptor A4 |
NCBI Official Symbol: | EPHA4 |
NCBI Official Synonym Symbols: | SEK; HEK8; TYRO1 |
NCBI Protein Information: | ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8 |
UniProt Protein Name: | Ephrin type-A receptor 4 |
UniProt Synonym Protein Names: | EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK |
Protein Family: | Ephrin type-A receptor |
UniProt Gene Name: | EPHA4 |
UniProt Entry Name: | EPHA4_HUMAN |
Antibodies | ELISA Kits |
Anti-EPHA4 Antibody (CAB8346) | Human EPHA4 / Eph receptor A4 ELISA Kit |
Phospho-EPHA4 (Y596) Antibody (PACO02651) | Human Ephrin type-A receptor 4 (EPHA4) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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