EP300 Antibody, FITC conjugated (PACO45804)
- SKU:
- PACO45804
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- FITC
Description
抗体名: | EP300 Antibody, FITC conjugated (PACO45804) |
抗体コード: | PACO45804 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human Histone acetyltransferase p300 protein (2283-2414AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | FITC |
バックグラウンド: | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity. Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Acetylates MEF2D. |
シノニム: | Histone acetyltransferase p300 (p300 HAT) (EC 2.3.1.48) (E1A-associated protein p300) (Histone butyryltransferase p300) (EC 2.3.1.-) (Histone crotonyltransferase p300) (EC 2.3.1.-) (Protein propionyltransferase p300) (EC 2.3.1.-), EP300, P300 |
UniProt Protein Function: | p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2. |
UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; EC 2.3.1.48; Nuclear receptor co-regulator; Acetyltransferase Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: cytoplasm; histone acetyltransferase complex; nucleoplasm; nucleus; transcription factor complex Molecular Function:acetyltransferase activity; androgen receptor binding; beta-catenin binding; chromatin binding; chromatin DNA binding; damaged DNA binding; DNA binding; histone acetyltransferase activity; lysine N-acetyltransferase activity; nuclear hormone receptor binding; p53 binding; protein binding; protein C-terminus binding; transcription activator binding; transcription coactivator activity; transcription factor binding; transferase activity, transferring acyl groups; zinc ion binding Biological Process: apoptosis; B cell differentiation; circadian rhythm; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA repair; establishment and/or maintenance of chromatin architecture; fat cell differentiation; G2/M transition of mitotic cell cycle; gene expression; heart development; innate immune response; internal peptidyl-lysine acetylation; internal protein amino acid acetylation; lung development; mitotic cell cycle; N-terminal peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; Notch signaling pathway; nucleotide-excision repair; organ morphogenesis; platelet formation; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of protein binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein amino acid acetylation; protein stabilization; regulation of cell cycle; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; response to estrogen stimulus; response to hypoxia; skeletal muscle development; somitogenesis; stimulatory C-type lectin receptor signaling pathway; transcription from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral reproduction Disease: Colorectal Cancer; Rubinstein-taybi Syndrome 1; Rubinstein-taybi Syndrome 2 |
NCBI Summary: | This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q09472 |
NCBI GenInfo Identifier: | 223590203 |
NCBI Gene ID: | 2033 |
NCBI Accession: | Q09472.2 |
UniProt Secondary Accession: | Q09472,B1AKC2, |
UniProt Related Accession: | Q09472 |
Molecular Weight: | 264,161 Da |
NCBI Full Name: | Histone acetyltransferase p300 |
NCBI Synonym Full Names: | E1A binding protein p300 |
NCBI Official Symbol: | EP300 |
NCBI Official Synonym Symbols: | p300; KAT3B; RSTS2 |
NCBI Protein Information: | histone acetyltransferase p300 |
UniProt Protein Name: | Histone acetyltransferase p300 |
UniProt Synonym Protein Names: | E1A-associated protein p300 |
Protein Family: | Histone acetyltransferase |
UniProt Gene Name: | EP300 |
UniProt Entry Name: | EP300_HUMAN |