EOMES Antibody (PACO55474)
- SKU:
- PACO55474
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | EOMES Antibody (PACO55474) |
抗体コード: | PACO55474 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
反応性: | Human, Rat |
免疫原: | Recombinant Human Eomesodermin homolog protein (562-676AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: 293 whole cell lysate, U251 whole cell lysate, Rat kidney tissue. All lanes: EOMES antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73, 48, 46, 75 kDa. Observed band size: 73 kDa. | |
IHC image of PACO55474 diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO55474 diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. |
シノニム: | Eomesodermin homolog (T-box brain protein 2) (T-brain-2) (TBR-2), EOMES, TBR2 |
UniProt Protein Function: | Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. |
NCBI Summary: | This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
UniProt Code: | O95936 |
NCBI GenInfo Identifier: | 229462982 |
NCBI Gene ID: | 8320 |
NCBI Accession: | O95936.3 |
UniProt Secondary Accession: | O95936,Q8TAZ2, Q9UPM7, B7Z4I2, B7ZA51, G3XAI5, |
UniProt Related Accession: | O95936 |
Molecular Weight: | 74,852 Da |
NCBI Full Name: | Eomesodermin homolog |
NCBI Synonym Full Names: | eomesodermin |
NCBI Official Symbol: | EOMES |
NCBI Official Synonym Symbols: | TBR2 |
NCBI Protein Information: | eomesodermin homolog |
UniProt Protein Name: | Eomesodermin homolog |
UniProt Synonym Protein Names: | T-box brain protein 2; T-brain-2; TBR-2 |
Protein Family: | Eomesodermin |
UniProt Gene Name: | EOMES |
UniProt Entry Name: | EOMES_HUMAN |