EDC4 Antibody (PACO57216)
- SKU:
- PACO57216
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | EDC4 Antibody (PACO57216) |
抗体コード: | PACO57216 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Enhancer of mRNA-decapping protein 4 protein (753-952AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO57216 diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of Hela cells with PACO57216 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO57216 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro). |
シノニム: | Enhancer of mRNA-decapping protein 4 (Autoantigen Ge-1) (Autoantigen RCD-8) (Human enhancer of decapping large subunit) (Hedls), EDC4, HEDLS |
UniProt Protein Function: | EDC4: a protein required for the 5' a?? 3' pathway of mRNA decapping and degradation-based post-transcriptional gene silencing. The proteins involved in 5' a?? 3' mRNA decay include DCP2, DCP1A, EDC3, EDC4 and DDX6, and are concentrated in cytoplasmic structures called mRNA "processing bodies" (P-bodies). Shortening of the 3'-poly(A) tail and removal of PABP precedes cleavage of the 5'-mRNA cap by a complex containing decapping enzymes 1A and 2 (DCP1A/DCP2). EDC4 enhances the catalytic activity of DCP2 in vitro. Shown to be required for silencing by miRNAs in Drosophila melanogaster. Two alternatively spliced human isoforms have been described. |
UniProt Protein Details: | Protein type:RNA processing Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol Molecular Function:protein binding Biological Process: gene expression; mRNA catabolic process, deadenylation-dependent decay |
UniProt Code: | Q6P2E9 |
NCBI GenInfo Identifier: | 74758241 |
NCBI Gene ID: | 23644 |
NCBI Accession: | Q6P2E9.1 |
UniProt Secondary Accession: | Q6P2E9,Q13025, Q13826, Q6ZR12, Q7Z6H7, A6NGM1, A8K4T4 |
UniProt Related Accession: | Q6P2E9 |
Molecular Weight: | 151,661 Da |
NCBI Full Name: | Enhancer of mRNA-decapping protein 4 |
NCBI Synonym Full Names: | enhancer of mRNA decapping 4 |
NCBI Official Symbol: | EDC4 |
NCBI Official Synonym Symbols: | GE1; Ge-1; RCD8; HEDL5; HEDLS; RCD-8 |
NCBI Protein Information: | enhancer of mRNA-decapping protein 4; autoantigen Ge-1; autoantigen RCD-8; human enhancer of decapping large subunit |
UniProt Protein Name: | Enhancer of mRNA-decapping protein 4 |
UniProt Synonym Protein Names: | Autoantigen Ge-1; Autoantigen RCD-8; Human enhancer of decapping large subunit |
Protein Family: | Enhancer of mRNA-decapping protein |
UniProt Gene Name: | EDC4 |
UniProt Entry Name: | EDC4_HUMAN |