DPM1 Antibody (PACO53010)
- SKU:
- PACO53010
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | DPM1 Antibody (PACO53010) |
抗体コード: | PACO53010 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Dolichol-phosphate mannosyltransferase subunit 1 protein (2-260AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Mouse lung tissue. All lanes: DPM1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 30 kDa. Observed band size: 30 kDa. |
![]() | Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO53010 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO53010 at dilution of 1:100. |
バックグラウンド: | Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins; catalytic subunit of the dolichol-phosphate mannose (DPM) synthase complex. |
シノニム: | Dolichol-phosphate mannosyltransferase subunit 1 (EC 2.4.1.83) (Dolichol-phosphate mannose synthase subunit 1) (DPM synthase subunit 1) (Dolichyl-phosphate beta-D-mannosyltransferase subunit 1) (Mannose-P-dolichol synthase subunit 1) (MPD synthase subunit 1), DPM1 |
UniProt Protein Function: | DPM1: Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O- mannosylation of proteins. Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E). CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features. Belongs to the glycosyltransferase 2 family. |
UniProt Protein Details: | Protein type:Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.83; Transferase Chromosomal Location of Human Ortholog: 20q13.13 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; nucleus Molecular Function:mannose binding; protein binding; alcohol binding; dolichyl-phosphate-mannose-protein mannosyltransferase activity; dolichyl-phosphate beta-D-mannosyltransferase activity Biological Process: cellular protein metabolic process; protein amino acid mannosylation; protein amino acid O-linked mannosylation; dolichol-linked oligosaccharide biosynthetic process; GDP-mannose metabolic process; GPI anchor biosynthetic process; C-terminal protein lipidation; dolichol metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification Disease: Congenital Disorder Of Glycosylation, Type Ie |
NCBI Summary: | Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq, Jul 2008] |
UniProt Code: | O60762 |
NCBI GenInfo Identifier: | 48145969 |
NCBI Gene ID: | 8813 |
NCBI Accession: | CAG33207.1 |
UniProt Secondary Accession: | O60762,O15157, Q6IB78, Q96HK0, |
UniProt Related Accession: | O60762 |
Molecular Weight: | 29,634 Da |
NCBI Full Name: | DPM1 |
NCBI Synonym Full Names: | dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit |
NCBI Official Symbol: | DPM1 |
NCBI Official Synonym Symbols: | MPDS; CDGIE |
NCBI Protein Information: | dolichol-phosphate mannosyltransferase subunit 1; DPM synthase complex, catalytic subunit; DPM synthase subunit 1; MPD synthase subunit 1; dolichol monophosphate mannose synthase; dolichol-phosphate mannose synthase subunit 1; dolichyl-phosphate beta-D-mannosyltransferase subunit 1; mannose-P-dolichol synthase subunit 1 |
UniProt Protein Name: | Dolichol-phosphate mannosyltransferase subunit 1 |
UniProt Synonym Protein Names: | Dolichol-phosphate mannose synthase subunit 1; DPM synthase subunit 1; Dolichyl-phosphate beta-D-mannosyltransferase subunit 1; Mannose-P-dolichol synthase subunit 1; MPD synthase subunit 1 |
Protein Family: | Dolichol-phosphate mannosyltransferase |
UniProt Gene Name: | DPM1 |
UniProt Entry Name: | DPM1_HUMAN |