DLK1 Antibody (PACO26249)
- SKU:
- PACO26249
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | DLK1 Antibody (PACO26249) |
抗体コード: | PACO26249 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human, Rat |
免疫原: | Recombinant Human Protein delta homolog 1 protein (24-303AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue All lanes: DLK1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 34 kDa Observed band size: 42 kDa . |
![]() | Immunofluorescence staining of HepG2 cells with PACO26249 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO26249 diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | May have a role in neuroendocrine differentiation. |
シノニム: | Protein delta homolog 1 (DLK-1) (pG2) [Cleaved into: Fetal antigen 1 (FA1)], DLK1, DLK |
UniProt Protein Function: | DLK1: May have a role in neuroendocrine differentiation. Monomer. Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 14q32 Cellular Component: extracellular space Biological Process: negative regulation of Notch signaling pathway |
NCBI Summary: | This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015] |
UniProt Code: | P80370 |
NCBI GenInfo Identifier: | 296439371 |
NCBI Gene ID: | 8788 |
NCBI Accession: | P80370.3 |
UniProt Secondary Accession: | P80370,P15803, Q96DW5, |
UniProt Related Accession: | P80370 |
Molecular Weight: | 33,067 Da |
NCBI Full Name: | Protein delta homolog 1 |
NCBI Synonym Full Names: | delta like non-canonical Notch ligand 1 |
NCBI Official Symbol: | DLK1 |
NCBI Official Synonym Symbols: | DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1 |
NCBI Protein Information: | protein delta homolog 1 |
UniProt Protein Name: | Protein delta homolog 1 |
UniProt Synonym Protein Names: | pG2 |
Protein Family: | Protein |
UniProt Gene Name: | DLK1 |
UniProt Entry Name: | DLK1_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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