CHODL Antibody (PACO59696)
- SKU:
- PACO59696
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | CHODL Antibody (PACO59696) |
抗体コード: | PACO59696 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Chondrolectin protein (105-206AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate. All lanes: CHODL antibody at 6.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 31, 26, 28, 29 kDa. Observed band size: 31 kDa. | |
Immunofluorescence staining of Hela cells with PACO59696 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO59696 diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | May play a role in the development of the nervous system such as in neurite outgrowth and elongation. May be involved in motor axon growth and guidance. |
シノニム: | Chondrolectin (Transmembrane protein MT75), CHODL, C21orf68 |
UniProt Protein Function: | May play a role in the development of the nervous system such as in neurite outgrowth and elongation. May be involved in motor axon growth and guidance. |
NCBI Summary: | This gene encodes a type I membrane protein with a carbohydrate recognition domain characteristic of C-type lectins in its extracellular portion. In other proteins, this domain is involved in endocytosis of glycoproteins and exogenous sugar-bearing pathogens. This protein localizes predominantly to the perinuclear region. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
UniProt Code: | Q9H9P2 |
NCBI GenInfo Identifier: | 20127636 |
NCBI Gene ID: | 140578 |
NCBI Accession: | NP_079220 |
UniProt Secondary Accession: | Q9H9P2,Q7Z798, Q7Z799, Q7Z7A0, Q9HCY3, B2R9C0, B4DJB8 |
UniProt Related Accession: | Q9H9P2 |
Molecular Weight: | 28,418 Da |
NCBI Full Name: | chondrolectin isoform a |
NCBI Synonym Full Names: | chondrolectin |
NCBI Official Symbol: | CHODL |
NCBI Official Synonym Symbols: | MT75; PRED12; C21orf68 |
NCBI Protein Information: | chondrolectin |
UniProt Protein Name: | Chondrolectin |
UniProt Synonym Protein Names: | Transmembrane protein MT75 |
Protein Family: | Chondrolectin |
UniProt Gene Name: | CHODL |
Antibodies |
Anti-CHODL Antibody (CAB17827) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |