CD82 Antibody (PACO56290)
- SKU:
- PACO56290
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | CD82 Antibody (PACO56290) |
抗体コード: | PACO56290 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human CD82 antigen protein (111-228AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO56290 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of A549 cells with PACO56290 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. |
シノニム: | CD82 antigen (C33 antigen) (IA4) (Inducible membrane protein R2) (Metastasis suppressor Kangai-1) (Suppressor of tumorigenicity 6 protein) (Tetraspanin-27) (Tspan-27) (CD antigen CD82), CD82, KAI1 SAR2 ST6 TSPAN27 |
UniProt Protein Function: | CD82: a widespread transmembrane protein of the tetraspanin family. A metastasis-suppressor whose decreased expression may be involved in malignant progression. Suppresses tumor metastasis of many cancers including cancers of the prostate, bladder, colon, cervix, liver, and lung (NSCLC). It is a target of estrogen receptor-mediated gene repression and is downregulated in primary human breast cancer. May be a prognostic marker for lung cancer and tumor metastatic potential. Interacts with cell surface proteins including integrins, cadherins, CD4, CD8, IGSF8. Modulates EGFR signaling. May suppress invasion by inhibiting integrin-dependent crosstalk with c-Met receptor and Src kinases. Its regulation of c-Met signaling apparently affects cancer cell migration. |
UniProt Protein Details: | Protein type:Cell surface; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Disease: Prostate Cancer |
NCBI Summary: | This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P27701 |
NCBI GenInfo Identifier: | 4504813 |
NCBI Gene ID: | 3732 |
NCBI Accession: | NP_002222 |
UniProt Secondary Accession: | P27701,Q7Z2D4, Q7Z5N2, D3DQN6, E9PC70, |
UniProt Related Accession: | P27701 |
Molecular Weight: | 26,818 Da |
NCBI Full Name: | CD82 antigen isoform 1 |
NCBI Synonym Full Names: | CD82 molecule |
NCBI Official Symbol: | CD82 |
NCBI Official Synonym Symbols: | R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27 |
NCBI Protein Information: | CD82 antigen; tspan-27; C33 antigen; tetraspanin-27; inducible membrane protein R2; metastasis suppressor Kangai-1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) |
UniProt Protein Name: | CD82 antigen |
UniProt Synonym Protein Names: | C33 antigen; IA4; Inducible membrane protein R2; Metastasis suppressor Kangai-1; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; CD_antigen: CD82 |
Protein Family: | CD82 antigen |
UniProt Gene Name: | CD82 |
UniProt Entry Name: | CD82_HUMAN |