Anti-ZO-1 Antibody (CAB0659)
- SKU:
- CAB0659
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ZO-1 Antibody |
| 抗体コード: | CAB0659 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human ZO-1 (NP_003248.3). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, HeLa, NIH/3T3, Mouse uterus, Rat liver |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human ZO-1 (NP_003248.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PKYQ INNI STVP KAIP VSPS AVEE DEDE DGHT VVAT ARGI FNSN GGVL SSIE TGVS IIIP QGAI PEGV EQEI YFKV CRDN SILP PLDK EKGE TLLS PLVM C |
| 遺伝子ID: | 7082 |
| Uniprot: | Q07157 |
| セルラーロケーション: | Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, gap junction, tight junction |
| 計算された分子量: | 186kDa/195kDa |
| 観察された分子量: | 250kDa |
| 同義語: | TJP1, ZO-1 |
| バックグラウンド: | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 15q13 Cellular Component: apical junction complex; intercellular canaliculus; tight junction; apicolateral plasma membrane; basolateral plasma membrane; cytosol; cell-cell adherens junction; apical part of cell; cytoplasm; apical plasma membrane; plasma membrane; gap junction; nucleus; cell junction Molecular Function:protein C-terminus binding; calmodulin binding; protein domain specific binding; protein binding Biological Process: response to drug; intercellular junction assembly; response to ethanol; sensory perception of sound; apoptosis; negative regulation of vascular permeability; blastocyst formation; response to lipopolysaccharide; cell structure disassembly during apoptosis |
| NCBI Summary: | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q07157 |
| NCBI GenInfo Identifier: | 85700443 |
| NCBI Gene ID: | 7082 |
| NCBI Accession: | Q07157.3 |
| UniProt Secondary Accession: | Q07157,Q2NKP3, Q4ZGJ6, B4E3K1, |
| UniProt Related Accession: | Q07157 |
| Molecular Weight: | 1748 |
| NCBI Full Name: | Tight junction protein ZO-1 |
| NCBI Synonym Full Names: | tight junction protein 1 |
| NCBI Official Symbol: | TJP1 |
| NCBI Official Synonym Symbols: | ZO-1 |
| NCBI Protein Information: | tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein |
| UniProt Protein Name: | Tight junction protein ZO-1 |
| UniProt Synonym Protein Names: | Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1 |
| UniProt Gene Name: | TJP1 |
| UniProt Entry Name: | ZO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZO-1 antibody (CAB0659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s. |
 | Western blot analysis of extracts of Rat liver, using ZO-1 antibody (CAB0659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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