Anti-ZC3HC1 Antibody (CAB6331)
- SKU:
- CAB6331
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-ZC3HC1 Antibody |
| 抗体コード: | CAB6331 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZC3HC1 (NP_057562.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZC3HC1 (NP_057562.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAP CEGQ AFAV GVEK NWGA VVRS PEGT PQKI RQLI DEGI APEE GGVD AKDT SATS QSVN GSPQ AEQP SLES TSKE AFFS RVET FSSL KWAG KPFE LSPL VCAK YGWV TVEC DMLK CSSC QAFL CASL QPAF DFDR YKQR CAEL KKAL CTAH EKFC FWPD SPSP DRFG MLPL DEPA ILVS EFLD RFQS LCHL DLQL PSLR PEDL KTMC LTED KISL LLHL LEDE LDHR TDER KTTI KLGS DIQV HVTA CILS VCGW ACSS |
| 遺伝子ID: | 51530 |
| Uniprot: | Q86WB0 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 47kDa/53kDa/55kDa |
| 観察された分子量: | 55kDa |
| 同義語: | ZC3HC1, HSPC216, NIPA |
| バックグラウンド: | This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. |
| UniProt Protein Function: | NIPA: a nuclear protein that interacts with anaplastic lymphoma kinase (ALK). An essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1. Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting cyclin B1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of cyclin B1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Three splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 7q32.2 Cellular Component: nuclear membrane; nucleus Molecular Function:protein binding; protein kinase binding; zinc ion binding Biological Process: cell division; mitosis; protein ubiquitination |
| NCBI Summary: | This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013] |
| UniProt Code: | Q86WB0 |
| NCBI GenInfo Identifier: | 73921220 |
| NCBI Gene ID: | 51530 |
| NCBI Accession: | Q86WB0.1 |
| UniProt Secondary Accession: | Q86WB0,Q75MF3, Q75MF4, Q8N330, Q96F75, Q9HA34, Q9NVX4 Q9P0R0, A6NH66, |
| UniProt Related Accession: | Q86WB0 |
| Molecular Weight: | 47,771 Da |
| NCBI Full Name: | Nuclear-interacting partner of ALK |
| NCBI Synonym Full Names: | zinc finger C3HC-type containing 1 |
| NCBI Official Symbol: | ZC3HC1 |
| NCBI Official Synonym Symbols: | NIPA; HSPC216 |
| NCBI Protein Information: | nuclear-interacting partner of ALK |
| UniProt Protein Name: | Nuclear-interacting partner of ALK |
| UniProt Synonym Protein Names: | Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1 |
| Protein Family: | NIPA-like protein |
| UniProt Gene Name: | ZC3HC1 |
| UniProt Entry Name: | NIPA_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using ZC3HC1 antibody (CAB6331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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