Anti-XRCC1 Antibody (CAB0442)
- SKU:
- CAB0442
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-XRCC1 Antibody |
| 抗体コード: | CAB0442 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human XRCC1 (NP_006288.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse liver, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human XRCC1 (NP_006288.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPEI RLRH VVSC SSQD STHC AENL LKAD TYRK WRAA KAGE KTIS VVLQ LEKE EQIH SVDI GNDG SAFV EVLV GSSA GGAG EQDY EVLL VTSS FMSP SESR SGSN PNRV RMFG PDKL VRAA AEKR WDRV KIVC SQPY SKDS PFGL SFVR FHSP PDKD EAEA PSQK VTVT KLGQ FRVK EEDE SANS LRPG ALFF SRIN KTSP VTAS DPAG PSYA AATL QASS AASS ASPV SRAI GSTS KPQE SPKG KRKL DLNQ EEKK TPSK PPAQ LSPS VPKR PKLP APTR TPAT APVP ARAQ GAVT GKPR GEGT EPRR PRAG PEEL GKIL |
| 遺伝子ID: | 7515 |
| Uniprot: | P18887 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 69kDa |
| 観察された分子量: | 69kDa |
| 同義語: | XRCC1, RCC |
| バックグラウンド: | The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
| UniProt Protein Function: | XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; enzyme binding; damaged DNA binding Biological Process: single strand break repair; base-excision repair; DNA repair |
| NCBI Summary: | The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P18887 |
| NCBI GenInfo Identifier: | 317373290 |
| NCBI Gene ID: | 7515 |
| NCBI Accession: | P18887.2 |
| UniProt Secondary Accession: | P18887,Q6IBS4, Q9HCB1, |
| UniProt Related Accession: | P18887 |
| Molecular Weight: | 69,477 Da |
| NCBI Full Name: | DNA repair protein XRCC1 |
| NCBI Synonym Full Names: | X-ray repair complementing defective repair in Chinese hamster cells 1 |
| NCBI Official Symbol: | XRCC1 |
| NCBI Official Synonym Symbols: | RCC |
| NCBI Protein Information: | DNA repair protein XRCC1; X-ray repair cross-complementing protein 1 |
| UniProt Protein Name: | DNA repair protein XRCC1 |
| UniProt Synonym Protein Names: | X-ray repair cross-complementing protein 1 |
| Protein Family: | DNA repair protein |
| UniProt Gene Name: | XRCC1 |
| UniProt Entry Name: | XRCC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using XRCC1 antibody (CAB0442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using XRCC1 antibody (CAB0442) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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