Anti-WHSC1L1 Antibody (CAB5577)
- SKU:
- CAB5577
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-WHSC1L1 Antibody |
| 抗体コード: | CAB5577 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WHSC1L1 (NP_060248.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, HepG2, HeLa, MCF7, Mouse liver, Mouse brain, Mouse spleen, Mouse kidney, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WHSC1L1 (NP_060248.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDFS FSFM QGIM GNTI QQPP QLID SANI RQED AFDN NSDI AEDG GQTP YEAT LQQG FQYP ATTE DLPP LTNG YPSS ISVY ETQT KYQS YNQY PNGS ANGF GAVR NFSP TDYY HSEI PNTR PHEI LEKP SPPQ PPPP PSVP QTVI PKKT GSPE IKLK ITKT IQNG RELF ESSL CGDL LNEV QASE HTKS KHES RKEK RKKS NKHD SSRS EERK SHKI PKLE PEEQ NRPN ERVD TVSE KPRE EPVL KEEA PVQP ILSS VPTT EVST GVKF QV |
| 遺伝子ID: | 54904 |
| Uniprot: | Q9BZ95 |
| セルラーロケーション: | Chromosome, Nucleus |
| 計算された分子量: | 72kDa/154kDa/156kDa/160kDa/161kDa |
| 観察された分子量: | 80-90kDa |
| 同義語: | NSD3, KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328 |
| バックグラウンド: | This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. |
| UniProt Protein Function: | WHSC1L1: is related to the Wolf-Hirschhorn syndrome candidate-1 protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Oncoprotein; Histone-binding; EC 2.1.1.43; Methyltransferase Chromosomal Location of Human Ortholog: 8p11.2 Cellular Component: nucleoplasm; chromosome; nucleus Molecular Function:protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific) Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; negative regulation of transcription, DNA-dependent Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015] |
| UniProt Code: | Q9BZ95 |
| NCBI GenInfo Identifier: | 74761342 |
| NCBI Gene ID: | 54904 |
| NCBI Accession: | Q9BZ95.1 |
| UniProt Secondary Accession: | Q9BZ95,Q6P2L6, |
| UniProt Related Accession: | Q9BZ95 |
| Molecular Weight: | 162kDa |
| NCBI Full Name: | Histone-lysine N-methyltransferase NSD3 |
| NCBI Synonym Full Names: | nuclear receptor binding SET domain protein 3 |
| NCBI Official Symbol: | NSD3 |
| NCBI Official Synonym Symbols: | KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328 |
| NCBI Protein Information: | histone-lysine N-methyltransferase NSD3 |
| UniProt Protein Name: | Histone-lysine N-methyltransferase NSD3 |
| UniProt Synonym Protein Names: | Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1; WHSC1-like protein 1 |
| UniProt Gene Name: | WHSC1L1 |
| UniProt Entry Name: | NSD3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WHSC1L1 antibody (CAB5577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 cells using WHSC1L1 antibody (CAB5577). Blue: DAPI for nuclear staining. |
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