Anti-WDR82 Antibody (CAB15518)
- SKU:
- CAB15518
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-WDR82 Antibody |
| 抗体コード: | CAB15518 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 174-313 of human WDR82 (NP_079498.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, Mouse thymus, Mouse brain, Mouse heart, Mouse kidney, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 174-313 of human WDR82 (NP_079498.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | YDLR SFDK GPFA TFKM QYDR TCEW TGLK FSND GKLI LIST NGSF IRLI DAFK GVVM HTFG GYAN SKAV TLEA SFTP DSQF IMIG SEDG KIHV WNGE SGIK VAVL DGKH TGPI TCLQ FNPK FMTF ASAC SNMA FWLP TIDD |
| 遺伝子ID: | 80335 |
| Uniprot: | Q6UXN9 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 35kDa |
| 観察された分子量: | 35kDa |
| 同義語: | WDR82, MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WDR82A |
| バックグラウンド: |
| UniProt Protein Function: | WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS. |
| UniProt Protein Details: | Protein type:Methyltransferase Chromosomal Location of Human Ortholog: 3p21.2 Cellular Component: chromatin; histone methyltransferase complex; nuclear chromosome, telomeric region Molecular Function:histone lysine N-methyltransferase activity (H3-K4 specific); protein binding Biological Process: histone H3-K4 methylation |
| NCBI Summary: | TMEM113 (WDR82) is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes (Lee and Skalnik, 2005 [PubMed 16253997]; Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Jul 2010] |
| UniProt Code: | Q6UXN9 |
| NCBI GenInfo Identifier: | 74758580 |
| NCBI Gene ID: | 80335 |
| NCBI Accession: | Q6UXN9.1 |
| UniProt Secondary Accession: | Q6UXN9,Q8TEB2, A8K5R5, |
| UniProt Related Accession: | Q6UXN9 |
| Molecular Weight: | 35,079 Da |
| NCBI Full Name: | WD repeat-containing protein 82 |
| NCBI Synonym Full Names: | WD repeat domain 82 |
| NCBI Official Symbol: | WDR82 |
| NCBI Official Synonym Symbols: | SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047 |
| NCBI Protein Information: | WD repeat-containing protein 82 |
| UniProt Protein Name: | WD repeat-containing protein 82 |
| UniProt Synonym Protein Names: | Protein TMEM113; Swd2 |
| Protein Family: | WD repeat-containing protein |
| UniProt Gene Name: | WDR82 |
| UniProt Entry Name: | WDR82_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WDR82 antibody (CAB15518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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