Anti-WDFY2 Antibody (CAB10598)
- SKU:
- CAB10598
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-WDFY2 Antibody |
| 抗体コード: | CAB10598 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WDFY2 (NP_443182.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | NCI-H460, HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WDFY2 (NP_443182.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAE IQPK PLTR KPIL LQRM EGSQ EVVN MAVI VPKE EGVI SVSE DRTV RVWL KRDS GQYW PSVY HAMP SPCS CMSF NPET RRLS IGLD NGTI SEFI LSED YNKM TPVK NYQA HQSR VTMI LFVL ELEW VLST GQDK QFAW HCSE SGQR LGGY RTSA VASG LQFD VETR HVFI GDHS GQVT ILKL EQEN CTLV TTFR GHTG |
| 遺伝子ID: | 115825 |
| Uniprot: | Q96P53 |
| セルラーロケーション: | Cytoplasm, Early endosome, Endosome |
| 計算された分子量: | 45kDa |
| 観察された分子量: | 45kDa |
| 同義語: | WDFY2, PROF, WDF2, ZFYVE22 |
| バックグラウンド: | This gene encodes a protein that contains two WD domains and an FYVE zinc finger region. The function of this gene is unknown. An alternatively spliced transcript variant of this gene may exist. |
| UniProt Protein Function: | WDFY2: Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2 (PubMed:17313651). Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1 (PubMed:18388859). Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates. Participates in transferrin receptor endocytosis (PubMed:16873553). {ECO:0000250|UniProtKB:Q8BUB4, ECO:0000269|PubMed:16873553, ECO:0000269|PubMed:17313651, ECO:0000269|PubMed:18388859}. Homodimer (PubMed:16792529). Interacts (via WD repeats 1- 3) with AKT1, AKT2, PRKCZ and PRKCI (PubMed:16792529). Interacts with VAMP2 (PubMed:17313651). Forms a complex with VAMP2 and PRKCZ (PubMed:17313651). Interacts with FOXO1. Forms a complex with AKT1 and FOXO1. {ECO:0000250|UniProtKB:Q8BUB4, ECO:0000269|PubMed:16792529, ECO:0000269|PubMed:17313651} |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 13q14.3 Cellular Component: early endosome; vesicle Molecular Function:protein binding Biological Process: positive regulation of fat cell differentiation; positive regulation of protein amino acid phosphorylation |
| NCBI Summary: | This gene encodes a protein that contains two WD domains and an FYVE zinc finger region. The function of this gene is unknown. An alternatively spliced transcript variant of this gene may exist. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96P53 |
| NCBI GenInfo Identifier: | 51316902 |
| NCBI Gene ID: | 115825 |
| NCBI Accession: | Q96P53.2 |
| UniProt Secondary Accession: | Q96P53,Q96CS1, B1AL86, |
| UniProt Related Accession: | Q96P53 |
| Molecular Weight: | 45kDa |
| NCBI Full Name: | WD repeat and FYVE domain-containing protein 2 |
| NCBI Synonym Full Names: | WD repeat and FYVE domain containing 2 |
| NCBI Official Symbol: | WDFY2 |
| NCBI Official Synonym Symbols: | PROF; WDF2; ZFYVE22 |
| NCBI Protein Information: | WD repeat and FYVE domain-containing protein 2 |
| UniProt Protein Name: | WD repeat and FYVE domain-containing protein 2 |
| UniProt Synonym Protein Names: | Propeller-FYVE protein |
| UniProt Gene Name: | WDFY2 |
 | Western blot analysis of extracts of various cell lines, using WDFY2 antibody (CAB10598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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