Anti-VRK1 Antibody (CAB7745)
- SKU:
- CAB7745
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-VRK1 Antibody |
| 抗体コード: | CAB7745 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF-7, Jurkat, A-549, HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GHLP WEDN LKDP KYVR DSKI RYRE NIAS LMDK CFPE KNKP GEIA KYME TVKL LDYT EKPL YENL RDIL LQGL KAIG SKDD GKLD LSVV ENGG LKAK TITK KRKK EIEE SKEP GVED TEWS NTQT EEAI QTRS RTRK RVQK |
| 遺伝子ID: | 7443 |
| Uniprot: | Q99986 |
| セルラーロケーション: | Cytoplasm, Nucleus, cytoskeleton, spindle |
| 計算された分子量: | 45kDa |
| 観察された分子量: | 45kDa |
| 同義語: | VRK1, PCH1, PCH1A |
| バックグラウンド: | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. |
| UniProt Protein Function: | VRK1: Serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA). Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor); CK1 group; VRK family Chromosomal Location of Human Ortholog: 14q32 Cellular Component: cytoplasm; cytosol; Golgi stack; nucleolus; nucleoplasm; nucleus Molecular Function:histone serine kinase activity (H3-S10 specific); nucleosomal histone binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity Biological Process: mitotic nuclear envelope disassembly; mitotic nuclear envelope reassembly; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell shape Disease: Pontocerebellar Hypoplasia, Type 1a |
| NCBI Summary: | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99986 |
| NCBI GenInfo Identifier: | 45593726 |
| NCBI Gene ID: | 7443 |
| NCBI Accession: | Q99986.1 |
| UniProt Secondary Accession: | Q99986,Q3SYL2, |
| UniProt Related Accession: | Q99986 |
| Molecular Weight: | 45,476 Da |
| NCBI Full Name: | Serine/threonine-protein kinase VRK1 |
| NCBI Synonym Full Names: | vaccinia related kinase 1 |
| NCBI Official Symbol: | VRK1 |
| NCBI Official Synonym Symbols: | PCH1; PCH1A |
| NCBI Protein Information: | serine/threonine-protein kinase VRK1 |
| UniProt Protein Name: | Serine/threonine-protein kinase VRK1 |
| UniProt Synonym Protein Names: | Vaccinia-related kinase 1 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | VRK1 |
| UniProt Entry Name: | VRK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VRK1 antibody (CAB7745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat lung using VRK1 antibody (CAB7745) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using VRK1 antibody (CAB7745) at dilution of 1:100 (40x lens). |
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