Anti-VPS4A Antibody (CAB7096)
- SKU:
- CAB7096
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-VPS4A Antibody |
| 抗体コード: | CAB7096 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, HeLa, U-87MG, SW620, A673, Mouse brain, Mouse testis, Mouse skeletal muscle, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTTS TLQK AIDL VTKA TEED KAKN YEEA LRLY QHAV EYFL HAIK YEAH SDKA KESI RAKC VQYL DRAE KLKD YLRS KEKH GKKP VKEN QSEG KGSD SDSE GDNP EKKK LQEQ LMGA VVME |
| 遺伝子ID: | 27183 |
| Uniprot: | Q9UN37 |
| セルラーロケーション: | Late endosome membrane, Midbody, Peripheral membrane protein, Prevacuolar compartment membrane |
| 計算された分子量: | 48kDa |
| 観察された分子量: | 49kDa |
| 同義語: | VPS4A, SKD1, SKD1A, SKD2, VPS4, VPS4-1 |
| バックグラウンド: | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. |
| UniProt Protein Function: | VPS4A: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis. Belongs to the AAA ATPase family. |
| UniProt Protein Details: | Protein type:EC 3.6.4.6; Vesicle Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: spindle pole; centrosome; late endosome membrane; perinuclear region of cytoplasm; cytoplasm; midbody; cytosol; nucleus Molecular Function:protein C-terminus binding; protein domain specific binding; protein binding; ATPase activity, coupled; microtubule-severing ATPase activity; ATP binding Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cell separation during cytokinesis; viral reproduction; cytokinesis; viral infectious cycle; endosome transport; mitotic metaphase plate congression; negative regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; vesicle-mediated transport; protein transport; abscission; nuclear organization and biogenesis; vacuole organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UN37 |
| NCBI GenInfo Identifier: | 62511240 |
| NCBI Gene ID: | 27183 |
| NCBI Accession: | Q9UN37.1 |
| UniProt Secondary Accession: | Q9UN37,Q793F9, Q8VEJ9, |
| UniProt Related Accession: | Q9UN37 |
| Molecular Weight: | 437 |
| NCBI Full Name: | Vacuolar protein sorting-associated protein 4A |
| NCBI Synonym Full Names: | vacuolar protein sorting 4 homolog A (S. cerevisiae) |
| NCBI Official Symbol: | VPS4A |
| NCBI Official Synonym Symbols: | SKD1; SKD2; VPS4; SKD1A; VPS4-1 |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 4A; VPS4A; hVPS4; SKD1-homolog; vacuolar sorting protein 4; vacuolar protein sorting factor 4A |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 4A |
| UniProt Synonym Protein Names: | Protein SKD2; VPS4-1; hVPS4 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS4A |
| UniProt Entry Name: | VPS4A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VPS4A antibody (CAB7096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using VPS4A antibody (CAB7096) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung using VPS4A antibody (CAB7096) at dilution of 1:100 (40x lens). |
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