Anti-VPS37A Antibody (CAB14159)
- SKU:
- CAB14159
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-VPS37A Antibody |
| 抗体コード: | CAB14159 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-397 of human VPS37A (NP_689628.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, HepG2, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-397 of human VPS37A (NP_689628.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPDV PDAF PELS ELSV SQLT DMNE QEEV LLEQ FLTL PQLK QIIT DKDD LVKS IEEL ARKN LLLE PSLE AKRQ TVLD KYEL LTQM KSTF EKKM QRQH ELSE SCSA SALQ ARLK VAAH EAEE ESDN IAED FLEG KMEI DDFL SSFM EKRT ICHC RRAK EEKL QQAI AMHS QFHA PL |
| 遺伝子ID: | 137492 |
| Uniprot: | Q8NEZ2 |
| セルラーロケーション: | Late endosome membrane, Nucleus, Peripheral membrane protein |
| 計算された分子量: | 20kDa/41kDa/44kDa |
| 観察された分子量: | 44kDa |
| 同義語: | VPS37A, HCRP1, PQBP2, SPG53 |
| バックグラウンド: | This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | VPS37A: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. Defects in VPS37A are a cause of autosomal recessive complex hereditary spastic paraparesis, a complicated form of hereditary spastic paraplegia (HSP). HSP is a heterogeneous group of neurodegenerative disorders characterized by progressive lower limb spasticity, retrograde degeneration of the crossed cortico- spinal tracts and thinning of the posterior columns in the spinal cord. Complicated forms are characterized by the addition of such neurological features as spastic quadriparesis, seizures, dementia, amyotrophy, extrapyramidal disturbance, cerebral or cerebellar atrophy, optic atrophy, and peripheral neuropathy, as well as by extra neurological manifestations such as dysmorphism, albinism, retinitis pigmentosa, deafness, dementia, amyotrophy and ichthyosis. Individuals with VPS37A mutations present with pronounced early onset spastic paraparesis of upper and lower limbs, mild intellectual disability, kyphosis, pectus carinatum and hypertrichosis. Belongs to the VPS37 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 8p22 Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; late endosome membrane; cytoplasm; endosome membrane Biological Process: protein transport; viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral protein processing; viral infectious cycle; virus assembly; endosome transport Disease: Spastic Paraplegia 53, Autosomal Recessive |
| NCBI Summary: | This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012] |
| UniProt Code: | Q8NEZ2 |
| NCBI GenInfo Identifier: | 74715446 |
| NCBI Gene ID: | 137492 |
| NCBI Accession: | Q8NEZ2.1 |
| UniProt Related Accession: | Q8NEZ2 |
| Molecular Weight: | 44kDa |
| NCBI Full Name: | Vacuolar protein sorting-associated protein 37A |
| NCBI Synonym Full Names: | VPS37A subunit of ESCRT-I |
| NCBI Official Symbol: | VPS37A |
| NCBI Official Synonym Symbols: | HCRP1; PQBP2; SPG53 |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 37A |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 37A |
| UniProt Synonym Protein Names: | ESCRT-I complex subunit VPS37A; Hepatocellular carcinoma-related protein 1 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS37A |
| UniProt Entry Name: | VP37A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VPS37A antibody (CAB14159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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