Anti-VPS35 Antibody (CAB7117)
- SKU:
- CAB7117
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-VPS35 Antibody |
| 抗体コード: | CAB7117 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, K-562, Mouse liver, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPTT QQSP QDEQ EKLL DEAI QAVK VQSF QMKR CLDK NKLM DALK HASN MLGE LRTS MLSP KSYY ELYM AISD ELHY LEVY LTDE FAKG RKVA DLYE LVQY AGNI IPRL YLLI TVGV VYVK SFPQ SRKD ILKD LVEM CRGV QHPL RGLF LRNY LLQC TRNI LPDE GEPT DEET TGDI SDSM DFVL LNFA EMNK LWVR MQHQ GHSR DREK RERE RQEL RILV GTNL VRLS QLEG VNVE RYKQ IVLT GILE QV |
| 遺伝子ID: | 55737 |
| Uniprot: | Q96QK1 |
| セルラーロケーション: | Cytoplasm, Early endosome, Endosome, Late endosome, Membrane, Peripheral membrane protein |
| 計算された分子量: | 91kDa |
| 観察された分子量: | 92kDa |
| 同義語: | VPS35, MEM3, PARK17 |
| バックグラウンド: | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. |
| UniProt Protein Function: | VPS35: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Defects in VPS35 are the cause of Parkinson disease type 17 (PARK17). PARK17 is an autosomal dominant, adult- onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Belongs to the VPS35 family. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 16q12 Cellular Component: retromer complex; lysosomal membrane; early endosome; late endosome; cytosol; endosome Molecular Function:protein binding; protein transporter activity Biological Process: intracellular protein transport; transcytosis; retrograde transport, endosome to Golgi Disease: Parkinson Disease 17 |
| NCBI Summary: | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96QK1 |
| NCBI GenInfo Identifier: | 25453321 |
| NCBI Gene ID: | 55737 |
| NCBI Accession: | Q96QK1.2 |
| UniProt Secondary Accession: | Q96QK1,Q561W2, Q9H016, Q9H096, Q9H4P3, Q9H8J0, Q9NRS7 Q9NVG2, Q9NX80, Q9NZK2, |
| UniProt Related Accession: | Q96QK1 |
| Molecular Weight: | 92kDa |
| NCBI Full Name: | Vacuolar protein sorting-associated protein 35 |
| NCBI Synonym Full Names: | vacuolar protein sorting 35 homolog (S. cerevisiae) |
| NCBI Official Symbol: | VPS35 |
| NCBI Official Synonym Symbols: | MEM3; PARK17 |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 35; hVPS35; maternal-embryonic 3 |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 35 |
| UniProt Synonym Protein Names: | Maternal-embryonic 3; Vesicle protein sorting 35 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS35 |
| UniProt Entry Name: | VPS35_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VPS35 antibody (CAB7117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
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