Anti-VAT1 Antibody (CAB17077)[KO Validated]

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SKU:
CAB17077
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

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抗体名:Anti-VAT1 Antibody [KO Validated]
抗体コード:CAB17077
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthetic peptide of human VAT1.
申し込み:WB IF
推奨希釈:WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:K-562, U-87MG, 293T
免疫原:A synthetic peptide of human VAT1.
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:Email for sequence
遺伝子ID:10493
Uniprot:Q99536
セルラーロケーション:
計算された分子量:28kDa/34kDa/41kDa
観察された分子量:42kDa
同義語:VAT1, VATI
バックグラウンド:Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins.
UniProt Protein Function:Possesses ATPase activity. Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
NCBI Summary:Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q99536
NCBI GenInfo Identifier:119581322
NCBI Gene ID:10493
NCBI Accession:EAW60918.1
UniProt Secondary Accession:Q99536,Q13035, Q5BKZ7, Q96A39, Q9BUT8, A8K345, B0AZP7 B4DPX4,
UniProt Related Accession:Q99536
Molecular Weight:28,341 Da
NCBI Full Name:vesicle amine transport protein 1 homolog (T californica), isoform CRA_a
NCBI Synonym Full Names:vesicle amine transport 1
NCBI Official Symbol:VAT1  
NCBI Official Synonym Symbols:VATI  
NCBI Protein Information:synaptic vesicle membrane protein VAT-1 homolog
UniProt Protein Name:Synaptic vesicle membrane protein VAT-1 homolog
Protein Family:Synaptic vesicle membrane protein
UniProt Gene Name:VAT1  
Anti-VAT1 Antibody [KO Validated] (CAB17077)Western blot analysis of extracts of various cell lines, using VAT1 antibody (CAB17077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
Anti-VAT1 Antibody [KO Validated] (CAB17077)Western blot analysis of extracts from normal (control) and VAT1 knockout (KO) 293T cells, using VAT1 antibody (CAB17077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-VAT1 Antibody [KO Validated] (CAB17077)Immunofluorescence analysis of L929 cells using VAT1 antibody (CAB17077) at dilution of 1:100. Blue: DAPI for nuclear staining.
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