Anti-USP25 Antibody (CAB12588)

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SKU:
CAB12588
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
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Description

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抗体名:Anti-USP25 Antibody
抗体コード:CAB12588
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3).
申し込み:WB IHC
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:HL-60, HT-1080, U-251MG, HeLa, SKOV3, Mouse testis, Rat testis
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MTVE QNVL QQSA AQKH QQTF LNQL REIT GIND TQIL QQAL KDSN GNLE LAVA FLTA KNAK TPQQ EETT YYQT ALPG NDRY ISVG SQAD TNVI DLTG DDKD DLQR AIAL SLAE SNRA FRET GITD EEQA ISRV LEAS IAEN KACL KRTP TEVW RDSR NPYD RKRQ DKAP VGLK NVGN TCWF SAVI QSLF NLLE FRRL VLNY KPPS NAQD LPRN QKEH RNLP FMRE LRYL FA
遺伝子ID:29761
Uniprot:Q9UHP3
セルラーロケーション:Cytoplasm, Nucleus
計算された分子量:122kDa/125kDa/129kDa
観察された分子量:130kDa
同義語:USP25, USP21
バックグラウンド:
UniProt Protein Function:USP25: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Protease; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 21q11.2

Cellular Component: cytoplasm; nucleus

Molecular Function:peptidase activity; protein binding; SUMO binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein modification process; proteolysis

NCBI Summary:Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9UHP3
NCBI GenInfo Identifier:302393833
NCBI Gene ID:29761
NCBI Accession:Q9UHP3.4
UniProt Secondary Accession:Q9UHP3,Q6DHZ9, Q9H9W1, C0LSZ0,
UniProt Related Accession:Q9UHP3
Molecular Weight:1055
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase 25
NCBI Synonym Full Names:ubiquitin specific peptidase 25
NCBI Official Symbol:USP25  
NCBI Official Synonym Symbols:USP21  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 25; USP on chromosome 21; ubiquitin thioesterase 25; deubiquitinating enzyme 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 25
UniProt Synonym Protein Names:Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25
UniProt Gene Name:USP25  
UniProt Entry Name:UBP25_HUMAN
Anti-USP25 Antibody (CAB12588)Western blot analysis of extracts of various cell lines, using USP25 antibody (CAB12588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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