Anti-USP2 Antibody (CAB10399)
- SKU:
- CAB10399
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-USP2 Antibody |
| 抗体コード: | CAB10399 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-430 of human USP2 (NP_004196.4). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | DU145, MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-430 of human USP2 (NP_004196.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ENYG RKGS ASQV PSQA PPSR VPEI ISPT YRPI GRYT LWET GKGQ APGP SRSS SPGR DGMN SKSA QGLA GLRN LGNT CFMN SILQ CLSN TREL RDYC LQRL YMRD LHHG SNAH TALV EEFA KLIQ TIWT SSPN DVVS PSEF KTQI QRYA PRFV GYNQ QDAQ EFLR FLLD GLHN EVNR VTLR PKSN PENL DHLP DDEK GRQM WRKY LERE DSRI GDLF VGQL KSSL TCTD CGY |
| 遺伝子ID: | 9099 |
| Uniprot: | O75604 |
| セルラーロケーション: | Cytoplasm, Nucleus, perinuclear region |
| 計算された分子量: | 40kDa/41kDa/45kDa/68kDa |
| 観察された分子量: | 72kDa |
| 同義語: | USP2, UBP41, USP9 |
| バックグラウンド: | This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease; Protease; EC 3.4.19.12 Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: centrosome; perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus Molecular Function:identical protein binding; protein binding; cyclin binding; ubiquitin protein ligase binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of skeletal muscle development; muscle development; protein deubiquitination; protein stabilization; positive regulation of skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; cell cycle; circadian behavior; entrainment of circadian clock by photoperiod; locomotor rhythm; circadian regulation of gene expression |
| NCBI Summary: | This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| UniProt Code: | O75604 |
| NCBI GenInfo Identifier: | 20141855 |
| NCBI Gene ID: | 9099 |
| NCBI Accession: | O75604.2 |
| UniProt Secondary Accession: | O75604,Q8IUM2, Q8IW04, Q96MB9, Q9BQ21, B0YJB8, E9PPM2 |
| UniProt Related Accession: | O75604 |
| Molecular Weight: | 605 |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 2 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 2 |
| NCBI Official Symbol: | USP2 |
| NCBI Official Synonym Symbols: | USP9; UBP41 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 2; ubiquitin thioesterase 2; deubiquitinating enzyme 2; ubiquitin specific protease 9; ubiquitin specific protease 12; 41 kDa ubiquitin-specific protease; ubiquitin-specific-processing protease 2 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 2 |
| UniProt Synonym Protein Names: | 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2 |
| Protein Family: | Protein |
| UniProt Gene Name: | USP2 |
| UniProt Entry Name: | UBP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP2 antibody (CAB10399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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