Anti-USP1 Antibody (CAB6785)
- SKU:
- CAB6785
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-USP1 Antibody |
| 抗体コード: | CAB6785 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 486-785 of human USP1 (NP_003359.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, HepG2, MCF7, Mouse testis, Mouse spleen, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 486-785 of human USP1 (NP_003359.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RWAI SQFA SVER IVGE DKYF CENC HHYT EAER SLLF DKMP EVIT IHLK CFAA SGLE FDCY GGGL SKIN TPLL TPLK LSLE EWST KPTN DSYG LFAV VMHS GITI SSGH YTAS VKVT DLNS LELD KGNF VVDQ MCEI GKPE PLNE EEAR GVVE NYND EEVS IRVG GNTQ PSKV LNKK NVEA IGLL GGQK SKAD YELY NKAS NPDK VAST AFAE NRNS ETSD TTGT HESD RNKE SSDQ TGIN ISGF ENKI SYVV QSLK EYEG KWLL FDDS EVKV TEEK DFLN SLSP STSP TSTP YLLF YKKL |
| 遺伝子ID: | 7398 |
| Uniprot: | O94782 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 88kDa |
| 観察された分子量: | 115kDa |
| 同義語: | USP1, UBP |
| バックグラウンド: | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. |
| UniProt Protein Function: | USP1: Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Interacts with FANCD2 and PCNA. Interacts with WDR48. Down-regulated following DNA damage. Belongs to the peptidase C19 family. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease; Protease Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: regulation of DNA repair; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA repair; skeletal development; response to UV |
| NCBI Summary: | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O94782 |
| NCBI GenInfo Identifier: | 12643657 |
| NCBI Gene ID: | 7398 |
| NCBI Accession: | O94782.1 |
| UniProt Secondary Accession: | O94782,Q05BX7, Q59H66, Q9UFR0, Q9UNJ3, A0PJ95, D3DQ57 |
| UniProt Related Accession: | O94782 |
| Molecular Weight: | 785 |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 1 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 1 |
| NCBI Official Symbol: | USP1 |
| NCBI Official Synonym Symbols: | UBP |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 1; hUBP; ubiquitin thioesterase 1; deubiquitinating enzyme 1; ubiquitin thiolesterase 1; ubiquitin specific protease 1; ubiquitin carboxyl terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin-specific-processing protease 1 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 1 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 1; hUBP; Ubiquitin thioesterase 1; Ubiquitin-specific-processing protease 1 |
| Protein Family: | UDP-sugar pyrophosphorylase |
| UniProt Gene Name: | USP1 |
| UniProt Entry Name: | UBP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP1 antibody (CAB6785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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