Anti-USO1 Antibody (CAB2796)
- SKU:
- CAB2796
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-USO1 Antibody |
| 抗体コード: | CAB2796 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human USO1 |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human |
| ポジティブサンプル: | 22Rv1, BT-474, K-562, THP-1, 293T, A-549 |
| 免疫原: | A synthetic peptide of human USO1 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 8615 |
| Uniprot: | O60763 |
| セルラーロケーション: | Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, cytosol |
| 計算された分子量: | 107kDa/109kDa |
| 観察された分子量: | 115kDa |
| 同義語: | USO1, P115, TAP, VDP |
| バックグラウンド: | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Vesicle Chromosomal Location of Human Ortholog: 4q21.1 Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; nucleolus; cytosol Molecular Function:protein transporter activity Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; vesicle fusion with Golgi apparatus; transcytosis; mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| UniProt Code: | O60763 |
| NCBI GenInfo Identifier: | 143743108 |
| NCBI Gene ID: | 8615 |
| NCBI Accession: | O60763.2 |
| UniProt Related Accession: | O60763 |
| Molecular Weight: | |
| NCBI Full Name: | General vesicular transport factor p115 |
| NCBI Synonym Full Names: | USO1 vesicle transport factor |
| NCBI Official Symbol: | USO1 |
| NCBI Official Synonym Symbols: | TAP; VDP; P115 |
| NCBI Protein Information: | general vesicular transport factor p115 |
| UniProt Protein Name: | General vesicular transport factor p115 |
| UniProt Synonym Protein Names: | Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein |
| Protein Family: | Tripeptidyl aminopeptidase |
| UniProt Gene Name: | USO1 |
| UniProt Entry Name: | USO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USO1 antibody (CAB2796) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody (CAB2796) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using USO1 antibody (CAB2796) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using USO1 antibody (CAB2796) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using USO1 antibody (CAB2796). Blue: DAPI for nuclear staining. |
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