Anti-UCHL1 Antibody (CAB0148)
- SKU:
- CAB0148
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-UCHL1 Antibody |
| 抗体コード: | CAB0148 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 59-223 of human UCHL1 (NP_004172.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-549, SH-SY5Y, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 59-223 of human UCHL1 (NP_004172.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | HENF RKKQ IEEL KGQE VSPK VYFM KQTI GNSC GTIG LIHA VANN QDKL GFED GSVL KQFL SETE KMSP EDRA KCFE KNEA IQAA HDAV AQEG QCRV DDKV NFHF ILFN NVDG HLYE LDGR MPFP VNHG ASSE DTLL KDAA KVCR EFTE REQG EVRF SAVA LCKA A |
| 遺伝子ID: | 7345 |
| Uniprot: | P09936 |
| セルラーロケーション: | Cytoplasm, Endoplasmic reticulum membrane, Lipid-anchor |
| 計算された分子量: | 24kDa |
| 観察された分子量: | 25kDa |
| 同義語: | HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1, UCHL1 |
| バックグラウンド: | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. |
| UniProt Protein Function: | UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell development/differentiation; Ligase; Protease; EC 3.4.19.12 Chromosomal Location of Human Ortholog: 4p14 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol Molecular Function:ubiquitin binding; omega peptidase activity; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of MAP kinase activity; axon transport of mitochondrion; protein deubiquitination; axon target recognition; eating behavior; neuromuscular process; muscle fiber development; sensory perception of pain; adult walking behavior Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant |
| NCBI Summary: | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| UniProt Code: | P09936 |
| NCBI GenInfo Identifier: | 136681 |
| NCBI Gene ID: | 7345 |
| NCBI Accession: | P09936.2 |
| UniProt Secondary Accession: | P09936,Q4W5K6, Q71UM0, |
| UniProt Related Accession: | P09936 |
| Molecular Weight: | 223 |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase isozyme L1 |
| NCBI Synonym Full Names: | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| NCBI Official Symbol: | UCHL1 |
| NCBI Official Synonym Symbols: | NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase isozyme L1 |
| UniProt Synonym Protein Names: | Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1 |
| Protein Family: | Ubiquitin carboxyl-terminal hydrolase |
| UniProt Gene Name: | UCHL1 |
| UniProt Entry Name: | UCHL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UCHL1 antibody (CAB0148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using UCHL1 antibody (CAB0148). Blue: DAPI for nuclear staining. |
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